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Soluble proteins of white skeletal muscle tissue of 15 species of Sparidae were analysed. Speciesspecific electrophoretic and isoelectric focusing patterns were found. Some bands exhibited the same mobility at genus level or at subfamily level, others differed significantly.Considerable similarity was observed in the species of the genera Spurus, Pugellus and Diplodus. Significant differences in the protein bands were noted between the contained subfamilies Denticinae, Sparinae and Boopsinae, confirming the existence of three separate phyletic lines within the family Sparidae.This study has shown that in these species there is a similarity between classifications based on morphological data and those derived from biochemical studies.Variation within species can be corrected for by carrying out multiple inter-specific comparisons and determining the variance of the similarity coefficients. Closely related species have similar patterns and, thus, higher similarity coefficients.The discrepancy in similarity matrices based on morphology and white skeletal muscle tissue proteins of sea bream species shows that electrophoretic methods provide additional information relevant to the systematics of fishes.Further work on comparison on soluble red muscle proteins of these species is proposed.

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Electrophoretic method has been applied to biochemical classification of hfugilids in the Lagoons of the Po delta. Among the various proteins analysed the one which turned out to be the most useful was the lactate dehydrogenase enzyme (LDH) because it presents different patterns in the five species living in the above mentioned lagoon and does not show any variations in the course of ontogenesis. This caracteristic makes it possible to identify stocks of fry to be put into closed waters for subsequent cultivation. hloreover this method allows one to make certain phylogenetic speculations with regard to the origin of these species.