The present study was designed to determine the effects of various levels of capsaicin (CAP) on endurance capacity in forty-nine male Sprague -Dawley rats, aged 4 weeks, which were assigned to four groups. Rats were given orally either control (0) or 6, 10 or 15 mg CAP/kg body weight 2 h before exercise by stomach intubations using a round-ended needle. The rats in each group were divided into two subgroups for resting or swimming exercise. Swimming exercise was performed with a weight corresponding to 3 % body weight attached to the tail, and endurance capacity was evaluated by swimming time to exhaustion. The highest dose (15 mg/kg) of CAP significantly (P, 0·05) increased endurance performance time and caused plasma non-esterified fatty acid to rise significantly by about 1·4-fold compared with that of non-CAP treated rats at exhaustion (P, 0·05). The highest dose of CAP had no effect on liver and gastrocnemius muscle glycogen (white and red) in resting rats, but significantly increased glycogen contents of both muscles at exhaustion (P,0·05). At rest, plasma noradrenalin levels of the rats receiving the highest dose were greater than that of non-CAP-treated rats and remained greater until exhaustion. The effects of CAP on endurance capacity have received little attention and have conveyed conflicting impressions. Kim et al. (1997) failed to show the maximal effect of 10 mg/kg doses of CAP on swimming endurance time in mice. The influences of various doses of CAP on endurance capacity were still unclear. Matsuo et al. (1996) reported that the intake of CAP have little sparing effect on glycogen in the liver and soleus muscles at rest and during exercise in rats previously fed a CAP-containing diet ad libitum for 1 week. Our present results suggest more than the highest dose of CAP (15 mg/kg) can cause the increase of endurance capacity, which might be induced through the sparing of muscle glycogen and the rise of non-esterfied fatty acids following the increase of circulating catecholamine.

This study was designed to determine whether combined treatments with genistein dosage and moderate resistance exercise would exhibit synergistically preventive effects on bone loss following the onset of menopause. Forty-one 12 wk-old female SD rats were assigned to five groups: 1) Sham operated (Sham); 2) ovariectomized (OVX-Cont); 3) OVX received genistein (OVX-GEN); 4) OVX exercised (OVX-EXE); and 5) OVX treated with both genistein and exercise (OVX-GEN-EXE). All rats were fed a low Ca (0.1%) diet ad libitum. Daily genistein dosage was 12 mg/kg body weight. Exercising rats took 40 sets of 1-min run interspersed with 1-min rest with a 100 g weight on the back on an uphill treadmill at 20 m/min. The experimental duration consisted of the adaptation and treatment periods of 4 weeks each. Uterine weight in OVX-Cont, OVX-GEN, OVX-EXE and OVX-GEN-EXE decreased to about 15% of that in Sham (p < 0.001). The femoral BMD (mg/cm2; mean +/- SE), assessed by DEXA (Lunar), of OVX-Cont was significantly lowered to 206 +/- 5 by -9%, as compared to 226 +/- 2 of Sham (p < 0.001). The BMD of OVX-GEN, OVX-EXE and OVX-GEN-EXE were 217 +/- 2, 217 +/- 2 and 222 +/- 2, respectively, and genistein dosage and resistance exercise equally increased the BMD of OVX rats by 5% (p < 0.01). Combined treatment of genistein and exercise more successfully recovered their decreased BMD by 8% (p < 0.001). BMD of the fourth lumbar vertebrae in OVX-Cont was declined to 191 +/- 7 by -15%, as compared to 225 +/- 4 in Sham (p < 0.001). OVX-EXE and OVX-GEN-EXE gained the BMD by 6% to 205 +/- 4 and 203 +/- 3, respectively, as compared to that of OVX-Cont (p < 0.01). These results suggest the possibility that the combined treatment of genistein dosage and resistance exercise have more beneficial effects by acting rather independently than their separate trials on the prevention of ovx-induced bone loss in femurs.

SummaryThe influences of various doses of capsaicin on endurance capacity remain to be clarified. The purpose of this study was to determine whether or not capsaicin delays stored tissue glycogen depletion. Rats were orally given either a vehicle or a dose of cap saicin, 6, 10, or 1.5mg/kg of body weight, 2h before exercise. The rats in each group were divided into three subgroups for resting and swimming exercise (30min, exhaustion). Swimming exercises were performed with a weight corresponding to 3% body weight at tached to the tail, and the endurance capacity was evaluated by the swimming time until exhaustion. The 15mg/kg dose of capsaicin significantly (p<0.05) enhanced the en durance performance time and plasma concentration of epinephrine, norepinephrine, free fatty acid and glucose rose to significantly higher levels within 30min; swimming exercise compared to rest (p<0.05). At the 15mg/kg capsaicin dosage, the plasma insulin level de creased to significantly lower levels in group subjected to 30-min swimming as compared to the resting group (p<0.05), while plasma glucagon rose to a significantly higher level (p<0.05). Liver and gastrocnemius muscle glycogen in the group subjected to 30-min swimming was maintained at significantly higher concentrations in the rats fed 15mg/kg of capsaicin as compared to the vehicle counterparts (p<0.05). These results suggest that the improvement in swimming endurance with the high capsaicin dosage is caused by an increase in fatty acid utilization as the energy source, resulting in the sparing of glycogen.

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The bioavailability for human intestinal bacteria of water-soluble dietary fiber preparations isolated from various cereals was examined. The effect of a hemicellulose preparation isolated from defatted rice bran (RBH) and high-methoxylated pectin (HMP) on fecal microflora and fermentation products in rats was also investigated. 1) Almost all the water-soluble dietary fiber preparations tested were rather resistant to bacterial degradation. RBH was simply degraded by Bifidobacterium compared with the other dietary fiber preparations. However, HMP was not degraded by the strains of intestinal bacteria used. 2) An increase in the fecal population of Bifidobacterium was shown in the dietary RBH group. The Bacteroidaceae population increased in the HMP group. The cecal pH value decreased in the dietary RBH and HMP groups, but was significantly lower in the former than in the latter. The addition of water-soluble dietary fiber to the diet resulted in an increase in the quantities of organic acids (mainly acetic acid) in the cecum and feces. These findings suggest that water-soluble dietary fiber, especially RBH, is effective for modifying colonic conditions.

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Stroke-prone spontaneously hypertensive rats (SHRSP) induce spontaneous osteoporosis. To elucidate the specific characteristics of bone metabolism, the SHRSP was compared with age matched Wistar-Kyoto (WKY) rats. We investigated the effects of prolonged swimming exercise training on bone mineral density (BMD) and metabolism in the SHRSP. Seven-week-old male SHRSP and WKY were divided into three groups; the sedentary control WKY group (n=6, WKY), the sedentary control SHRSP group (n=6, SP) and the swimming exercise training SHRSP group (n=6, SWIM) (in pool with 60 min./day, 5 days/week for 12 weeks). The femoral BMD, bone mineral content (BMC), strength, Ca and P contents (%) of SHRSP were approximately 17, 27, 25, 20 and 9%, respectively, lower than that of WKY (p<0.001). Serum alkaline phosphatase (AlP) had not changed between both of SP and WKY, but tartrate-resistant acid phosphatase (TrAcP) of SP approximately 3-fold higher than that of WKY (p<0.05). Both serum calcium (Ca) and intact parathyroid hormone (i-PTH) were similar between SP and WKY. However, serum phosphate (P) of SP was approximately 18% lower than that of WKY (N.S.). These results suggested that SHRSP induces osteopenia by the bone turnover of the promoted osteoclast activity with disturbed phosphate homeostasis. On the other hand, the femoral BMD and strength were approximately 7% and 20%, respectively, decreased in the SWIM (p<0.001), and f e mo ra l b on e C a an d P c o n t e n t s ( %) w e re a ls o approximately 11% and 14%, respectively, lower than that of SP (p<0.001). There were no significant difference between SWIM and SP on serum Ca, but serum P of SWIM was significantly lower than that of SP (p<0.05). These results suggested that the prolonged swimming exercise t r a i n i n g i n t h e S H R S P i n d u c e s m o r e c r u e l l y hypophosphatemia, and leading to osteopenia eventually. We conclude that SHRSP induces osteopenia with disturbance of phosphate homeostasis, and the prolonged swimming exercise in the SHRSP might deteriorate hypophosphatemia and osteopenia.