Selenium is an essential nutrient with diverse physiological functions, and soluble organic selenium (SOS) sources have a higher bioavailability than inorganic selenium sources. Based on the response surface methodology and central composite design, this study presents the optimal medium components for SOS accumulation in batch cultures of Flammulina velutipes, i.e. 30 g/L glucose, 11.2 mg/L sodium selenite, and 1.85 g/L NH 4 NO 3 . Furthermore, logistic function model feeding was found to be the optimal feeding strategy for SOS accumulation during Flammulina velutipes mycelia fermentation, where the maximum SOS accumulation reached (4.63 0.24) mg/L, which is consistent with the predicted value.
A method to estimate the content of selenium in organics was introduced based on reversed phase-high performance liquid chromatography (RP-HPLC). The maximum absorption peak of piazselenol was at 330 nm and the optimized temperature and pH value were 40 °C and 2.8, respectively. The minimum detection concentration of selenium(IV) was 0.06 μg/mL and the measurable range was 0.12 - 12.0 μg/mL. The organic selenium accumulation in golden needle mushroom (Flammulina velutipes) mycelia was obtained by subtracting the amount of inorganic selenium from that of total selenium. The organic selenium accumulation of various inoculation amounts showed that organic selenium accumulation in a unit volume of the fermentation broth was positively related the inoculation amount. Compared with the methods reported previously, the method used here is simple, reliable and less toxic
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