Oxyllen treatment of horse liver alcohol dchydro=cnase EE isoxymc sub.geStured with Ca(Ill at the catalytic site l.¢ads to bleaching with concomitant reduction to Call) of',.90~ of total Ca(ill, The Ca(Ill of the remaininll'minor species' cannot bc reduced nor does it intcraca with =oilrno~ liipmds. e,ll. 2-ntrrcaplorthanol, imida~ole, pyntzole, or ttxid¢ ions, The EPR spectrum is axial with a super.hyl~tl"ln¢ splitting of i S,6 (3 indicating binding of one nitrol~n atom to Cu(ll). There data as well as th¢ cncrl;ies and intensiti~ of the ~:bsorption and CD spa:tea SUli~t the Ca(H) ion of the minor species to tm located in the cat.dytic slt¢ of HLADH in a position and geometey different from that or the. major glories,
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