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Quantification of extractable antibiotics in soils is important to assessing their bioavailability and mobility, and ultimately their ecotoxicological and health risks. This study aimed to establish a biosensor method for detecting extractable tetracyclines in soils (Alfisol, Mollisol, and Ultisol) using whole-cell biosensors containing a reporter plasmid (pMTGFP or pMTmCherry) carrying fluorescent protein genes tightly controlled by tetracyclines-responsive control region (tetRO). This whole-cell biosensor method can simultaneously measure 96 or more samples within 6 h and is easily parallelizable, whereas a typical high-performance liquid chromatography (HPLC) method may require 7 times more of analysis time and much greater cost to achieve similar analytical throughput. The biosensor method had a detection limit for each of six tetracyclines between 5.32−10.2 μg/kg soil, which is considered adequate for detecting tetracyclines in ethylenediaminetetraacetic acid (EDTA) extracts of soils. Relative standard deviation was between 19.8−51.2% for the biosensor Escherichia coli DH5α/pMTGFP and 2.98−25.8% for E. coli DH5α/pMTmCherry, respectively, suggesting that E. coli DH5α/pMTmCherry was superior to E. coli DH5α/pMTGFP for detecting extractable tetracyclines in soils. This new, fast, easily parallelizable, and cost-effective biosensor method has the potential for measuring extractable concentrations of tetracyclines for a large number of soil samples in large-scale monitoring studies.

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On-site sanitation facilities, mostly pit latrines are the main points of human excreta disposal in periurban low-income settlements in Kenya. Collection, treatment and final disposal of pit latrine faecal sludge, pose a significant management problem and present public health risks. The choice of appropriate faecal sludge treatment technology depends on precise region based data on the sludge characteristics that are often unavailable. The study analysed physiochemical characteristics of faecal sludge sampled at different depths of pit latrines. Twenty-four samples were collected from six pit latrines along the depth strata at 1-m intervals from the surface to 3 m depth. Samples were analysed for chemical oxygen demand (COD), biochemical oxygen demand (BOD), ammonia, total nitrogen and total phosphorus. The mean COD: BOD ratio was 1:5 with a concentration of 112800 and 24600 mg/L, respectively. Concentrations for all parameters were variable and higher in comparison with properties reported in literature. Upper layers had higher concentrations than lower depths. The concentrations of the sludge were 10-100 higher than acceptable limits for in-fluent sludge into municipal wastewater treatment plants. These results show that disposal of pit latrine faecal sludge into the wastewater treatment plants without co-treatment overload the system since treatment plants in use currently have not been designed to handle pit latrine sludge. The properties of faecal sludge analysed indicate that the wastewater treatment plants may not be capable of treating faecal sludge unless co treatment mechanisms are applied. Therefore, influent faecal sludge must be maintained within allowable concentrations; otherwise, the effluents may lead to significant environmental pollution impacts.