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The influence of common ragweed (Ambrosia artemisiifoliaL.) interference on soybean [Glycine max(L.) Merr. ‘Ransom’] yield was studied in the field utilizing naturally occurring weed populations. The damage-threshold population for a full-season, in-row common ragweed infestation was four weeds/10 m of row, which resulted in an 8% yield loss. Soybeans kept weed-free for 2 weeks or longer after emergence in a dry year produced normal yields, but 4 weeks of weed-free maintenance was required when adequate moisture was available early in the growing season. Soybean yield was not reduced by a natural population of common ragweed if the period of interference was limited to 6 weeks or less after crop emergence. By 8 weeks after emergence, common ragweed height averaged 25 cm taller than soybeans, and the weed canopy intercepted 24% of the photosynthetically active radiation.

A clone has been isolated from a human lymphoblastic cDNA expression library that complements a mutant Chinese hamster cell defective in the uptake of the folate analogue methotrexate. When transfected with this clone the mutant cells regain the ability to transport the drug and, as a consequence, become sensitive to its cytotoxic action. The clone is 2863 base pairs long and has an open reading frame of 1770 base pairs that codes for a putative protein of 64 kDa. The putative protein has 51 and 50% identity at the amino acid level with the mouse and hamster functions, respectively, involved in the transport of reduced folates. Together these three proteins share 47% identity and have similar predicted structural features. The data are consistent with this human clone encoding either the reduced folate transporter or an auxiliary function that interacts with this transporter.

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