A procedure is presented for colorimetric analysis of starch and cellulose at a wave length of 625 mg, using a 0.1% solution of anthrone in concentrated sulfuric acid. The method is accurate for ranges of 10 to 200 micrograms and sensitive to 2 micrograms of these substances. Because of the instability of the reagent, a known standard must be used with each set of analyses to determine the correct Beer's law constant.Color intensity studies of the effect of heat upon the reaction between anthrone reagent and starch and cellulose are presented. Spectral transmittance curves of carbohvdrate-anthrone colors prepared under different conditions are also included.
LITERATURE CITED(1) Dreywood, Roman, Ind.
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