Summaryobjective To define the insecticide resistance status of Triatoma infestans to deltamethrin (pyrethroid), malathion (organophosphate) and bendiocarb (carbamate) in Bolivia.methods Fifty populations of T. infestans were sampled in Bolivian human dwellings. Quantal response data were obtained by topical applications of 0.2 ll of insecticide-acetone solutions on nymphs N1 of the F1 generations. For most populations, dose-mortality relationships and resistance ratios (RR) were analysed. Discriminating concentrations were established for each insecticide with a susceptible reference strain and used on the other field populations. A tarsal-contact diagnostic test using insecticide impregnated papers was designed to rapidly identify deltamethrin-resistant populations in the field.results Discriminating concentrations for topical applications were 5, 70 and 120 ng active ingredient per insect for deltamethrin, bendiocarb and malathion, respectively. The diagnostic concentration for deltamethrin was 0.30% for the 1-h exposure by tarsal contact. All populations sampled in human dwellings exhibited significant levels of resistance to deltamethrin, from 6 to 491 and varied among regions. Resistant populations did not recover complete susceptibility to deltamethrin when the synergist piperonyl butoxide (PBO) was used. None of the sampled populations exhibited significant resistance to bendiocarb (all RR 50 < 1.8) or malathion (all RR 50 < 2.2).conclusion In Bolivia, most 'domestic' T. infestans populations are resistant to deltamethrin. Because insecticide vector control is the only selection pressure, resistance likely originates from it. Switching from pyrethroids to organophosphates or carbamates could be a short-term solution to control this vector, but other alternative integrated control strategies should also be considered in the long term.

Background: The length of the gonotrophic cycle varies the vectorial capacity of a mosquito vector and therefore its exact estimation is important in epidemiological modelling. Because the gonotrophic cycle length depends on temperature, its estimation can be satisfactorily computed by means of physiological time analysis.

In ELISAs, sera of individuals infected by Trypanosoma cruzi show absorbance values above a cut-off value. The cut-off is generally computed by means of formulas that need absorbance readings of negative (and sometimes positive) controls, which are included in the titer plates amongst the unknown samples. When no controls are available, other techniques should be employed such as change-point analysis. The method was applied to Bolivian dog sera processed by ELISA to diagnose T. cruzi infection. In each titer plate, the change-point analysis estimated a step point which correctly discriminated among known positive and known negative sera, unlike some of the six usual cut-off formulas tested. To analyse the ELISAs results, the change-point method was as good as the usual cut-off formula of the form “mean + 3 standard deviation of negative controls”. Change-point analysis is therefore an efficient alternative method to analyse ELISA absorbance values when no controls are available.

BackgroundThe Human Blood Index (HBI, proportion of bloodmeals of a mosquito population obtained from man) is relevant to epidemiological assessment and to the modification of measures to interrupt malaria transmission since the vectorial capacity of the vector varies as the square of the HBI. Anopheles pseudopunctipennis is a main malaria vector in South America. Unfortunately, few data exist concerning HBI values in its range of distribution and none from Bolivia where this species is considered as an important malaria vector in the central Andes.MethodsThe host choice of An. pseudopunctipennis has been studied in Mataral, a characteristic village of the central Andes of Bolivia. Mosquito host feeding preference experiments (equal accessibility to host in homogenous environment) were monitored using baited mosquito nets in latin square designs. Host feeding selection experiments (natural feeding pattern in heterogeneous environment) was measured by bloodmeal analysis, using ELISA to determine the origin of blood. Mosquito bloodmeals were collected on various occasions, using various techniques in a variety of sampling sites. A survey of the possible blood sources has also been carried out in the village. Data were analysed with the forage ratio method.ResultsAn. pseudopunctipennis chooses amongst hosts. Sheep, goats, donkeys and humans are the preferred hosts, while dogs, pigs and chicken are rarely bitten. An. pseudopunctipennis has an opportunistic behaviour, in particular within the preferred hosts. The HBI in Mataral is ≈40% and in the central Andes, may range from 30–50%, in accordance to other findings. A high proportion of mixed meals were encountered (8%), and cryptic meals are likely more numerous. There was no difference amongst the HBI from parous and nulliparous mosquitoes.ConclusionForage ratio analysis is a powerful tool to interpret mosquito host choices. However, refinements in sampling strategies are still needed to derive accurate and precise HBIs that could be computed to compare or follow epidemiological situations. The low antropophily of An. pseudopunctipennis, associated with changing environmental conditions, leads to unstable malaria (Plasmodium vivax) transmission in the central Andes. The opportunistic behaviour of this vector may be used to attract mosquitoes to insecticide. Zooprophylaxis is a promising alternative control strategy.

BackgroundTriatoma infestans is the main vector of Chagas disease in the southern cone countries. Present control strategies based on indoor and outdoor residual insecticide spraying are not sufficient to control disease transmission, particularly in Bolivia. Techniques based on the management of the human environment may be good alternatives or supplements.MethodsSocial and entomological surveys were carried out in four villages of Bolivia situated in the dry inter-Andean Valleys and the Chaco region. Risk factors for house infestation by T. infestans were identified, and an eco-health intervention based on education and community participation was carried out to reduce the risks of house infestation. It consisted of implementing simple and low cost vector control techniques such as coating of mud walls, cleaning activities and removal of poultry that enter rooms to lay eggs.ResultsThe eco-health intervention significantly reduced the number of infested bedrooms, the mean abundance of T. infestans in bedrooms and beds, especially in the Chaco region. Mud wall coating was well accepted and could be proposed as a supplementary tool to the National Program of Chagas Disease Control to enhance the effects of insecticide sprayings.ConclusionsEven if cleaning activities were still neglected, community participation proved to be effective in reducing house infestation.