No abstract

Myocardial ischemic preconditioning (IPC) is a potent endogenous mechanism of cardioprotection against ischemia^reperfusion injury. In this study we focused on the second phase of IPC as the most interesting in terms of therapeutic implementations. We aimed at the detection of genes, which are di¡erentially expressed at 16 h after reperfusion. Preconditioning of canine myocardium was initiated by 5 min occlusion of the left anterior descending coronary artery with subsequent reperfusion. cDNA representational di¡erence analysis in combination with microarray hybridization and reverse transcription polymerase chain reaction were used to reveal the changes in gene expression in canine hearts. We found that functionally related genes for tristetraproline (TTP), selectin E, matrix metalloproteinase 9, and tumor necrosis factor-K K were highly upregulated at the late phase of IPC. The upregulation of TTP gene at the late phase of IPC, reported here for the ¢rst time, may represent a cardioprotective mechanism, which could be a promising perspective in clinical interventions against ischemia^reper-fusion injuries of the heart.

No abstract