Methods:The sample pretreatment involved protein precipitation with methanol-acetonitrile (50:50 by volume) followed by acidification with hydrochloric acid to convert the lactone ring-opened form into its lactone form, quantitatively. HPLC separation was performed on a Xterra RP18 column. The excitation wavelength was 370 nm, and the emission wavelength was set at 470 nm for the first 24 min and then at 534 nm for the next 4 min. The stabilities of irinotecan and its four metabolites in plasma, saliva, and acidic extracts were also investigated under various conditions. Results: Assays were linear in the tested range of 0.5-1000 g/L. For the five analytes, limits of quantification were 0.5 g/L in both matrices. The interassay imprecision (as relative standard deviation) was 3.2-14% in plasma and 2.6 -5.6% in saliva. Assay recoveries ranged from 92.8% to 111.2% for plasma and 100.1% to 104.1% for saliva. Mean extraction recovery from plasma or saliva was 90%.
A high-performance liquid chromatographic (HPLC) method for the determination of melphalan in human plasma was developed. This method involved a solid phase extraction (SPE) of melphalan and the internal standard (propylparaben) from plasma, using bond Elut C2 SPE ORDER REPRINTS columns with an elution solvent of 0.5 mL of acetonitrile-purified water (50 : 50, v/v). Separation of the two analytes was achieved within 15 min, using a reversed-phase Kromasil C18 analytical column (150 Â 4.6 mm I.D., 5 mm particle size) with a mobile phase of methanol -water -acetic acid (48 : 51 : 1, v/v). An ultraviolet detector operated at 261 nm was used, with a linear response observed from 10 to 250 ng mL 21 . Obtained from the method validation, inter-assay precision was below 6% and accuracy is near 100%. The extraction efficiency of the assay was approximately 71% and was constant across the calibration range. The lower limit of quantitation was 10 ng mL 21 ; at this level, precision was 5% and accuracy was 101%. The applicability of this method has been demonstrated by the successful analysis of clinical plasma samples. The SPE procedure developed in this paper, to quantify melphalan in biological samples requiring three steps for sample loading, clean-up, and elution, can easily be automated by using either a robot or an automated sample preparation system.
Oxaliplatin solutions diluted in 5% dextrose injection to 0.2 and 1.3 mg/mL were stable in PVC and PVC-free infusion bags for at least 14 days at both 4 degrees C and 20 degrees C without regard to light exposure.
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