This study aims to evaluate the production and quality of buffel (Pennisetum ciliare), rhodes (Chloris gayana), and blue grama (Bouteloua gracilis) forage with three defoliation dates —at 50, 80, and 110 days after regrowth (dar)— in Valle del Mezquital. Crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), total digestible nutrients (TDN), relative feed value (RFV), and metabolizable energy (ME) were determined. A completely randomized design with three repetitions, a Tukey test for mean separation (α 0.05), and SAS Proc GLM for data analysis were used. The highest forage production occurs in the rhodes forage at 110 dar (12,936 kg DM ha-1). The highest CP (10.6%) was found in the buffel forage at 50 dar; in the rhodes forage, both values (80 and 110) were lower than 7%. The highest RFV was obtained by exotic grasses; however, higher TDN was recorded for the blue grama grass. Rhodes grass obtained the lowest ME at 80 dar (1.76), while the highest ME was obtained by buffel at 50 dar (1.91). The three varieties can be defoliated when the plants show intermediate yield and nutritional value, i.e., 80 days after the regrowth begins; nevertheless, buffel and rhodes should be defoliated at 50 dar.
We tested whether periconceptional nutrition with cladodes from the cactus, Opuntia ficus-indica, with or without protein-enrichment, improved the metabolomic profile and reproductive outcomes of adult female sheep. Sixty Rambouillet ewes of similar body weight were randomly allocated among three nutritional treatments that were fed during the breeding period (34 days): Control (Control; n = 20), Opuntia (Opuntia; n = 20) and protein-enriched Opuntia (E-Opuntia; n = 20). There were no effects of treatment on body weight but assessment of urine samples indicated that, for 76 metabolites, the Control and Opuntia groups differed completely (P < 0.05), whereas there was overlap between the Control and E-Opuntia groups. It appears that, in Opuntia-fed and Control-fed sheep, different functional groups are activated leading to changes in the metabolism of glucose, tyrosine, methane, and glycerolipids. Fertility and reproductive rate tended to be higher in the Opuntia (70% and 95%) and E-Opuntia (90% and 110%) groups than in the Control (55% and 65%), and an orthogonal contrast revealed the difference between Control and Opuntia to be significant for both reproductive variables (P < 0.05). We conclude that nutritional supplementation with Opuntia cladodes, with or without protein enrichment, increased fertility rate and reproductive rate of female sheep, without any accompanying increases in body weight. Our observations suggest that the reproductive responses to Opuntia do not simply reflect a response to good nutrition, but might be caused by specific metabolites/metabolomic pathways, perhaps by an activation of the metabolism of glucose, methane, tyrosine and glycerolipids. There are few reports relating these metabolomic compounds with the metabolism of the sheep, let alone with reproductive efficiency. The novelty of these discoveries suggests that we need further research into the mechanisms through which nutrition affects the reproductive system.
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