Aim
To evaluate the distribution of bacteria in teeth with pulp necrosis and apical granuloma.
Materials and methods
Thirty-two extracted teeth with pulp necrosis and apical granulomas and 16 cases of isolated apical granulomas were sectioned and stained with Hematoxylin and Eosin (H&E) and Brown-Brenn staining for microscopic evaluation. The specimens were observed as to the location and distribution of bacteria involved in the process of pulp necrosis and their relationship with the periapical tooth structures and soft tissues.
Results
Stainable Gram-positive and Gram-negative bacteria were found in the entire root canal system. Bacterial biofilms were detected in 81.2% of cementum root canal, 46.8% in apical delta, 65.6% at the apical surface and 79.2% of the granulomas above all the specimens. Bacteria and bacterial biofilms were present in the entire root canal system of the infected teeth with apical granulomas.
Conclusion
Our findings suggest that bacterial infections can reach inaccessible areas to instrumentation.
How to cite this article
Ribeiro FC, Consolaro A, Pinheiro TN. Bacterial Distribution in Teeth with Pulp Necrosis and Apical Granuloma. Int J Experiment Dent Sci 2013;2(2):86-91.
The aim of this study was to evaluate the quality of root canal filling performed by undergraduate students at the Federal University of Espirito Santo (UFES). A total of 368 periapical radiographs of endodontic treatment performed by undergraduate students between 2012 and 2014 was assessment. The quality of root canal filling was based on two criteria: length of root canal filling and adequate density of the obturation. Root canal fillings were considered adequate if had no voids and were located at 0-2 mm below the radiographic apex. Statistical analysis was conducted using chi-square tests, with a 5% significance level. In 93.8% of the cases the root canal filling was considered adequate. The density of the obturation was considered inadequate in 3% of the cases. When the length of root canal filling was observed, 1.2% of cases were considered as over-filled and 2.2% were considered under-filled. Non-significant differences were observed among the tooth type (incisive, canines and pre-molars) and the quality of root canal filling. Most of root canal filling performed by undergraduate students at the UFES were considered adequate, regardless of tooth type.
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