RESUMO.-Atualmente os animais silvestres têm despertado o interesse particular na criação domestica. Na medicina de animais selvagens, os exames ultra-sonográficos podem ser considerados como ferramenta para diagnosticar e prevenir doenças. Deste modo, realizou-se um estudo em 20 jibóias (Boa constrictor), a fim de caracterizar a morfologia e aparência ultra-sonográfica das estruturas presentes da cavidade celomática desses animais. Ultra-sonograficamente, o fígado apresentou-se variando de hipoecóica a levemente hiperecogênica, com margens ecogênicas e ecotextura homogênea em toda sua extensão. Os rins mostraram formato elipsóide, com cápsula fina, regular e hiperecóica. Os folículos ovarianos apresentaram formato ovóide, margens finas, regulares e discretamente hiperecóicas. As estruturas do sistema reprodutor do macho não foram evidenciadas com precisão, devido a sua ecogenicidade similar em relação às Currently, wild animals have been received special attention for domestic breeding. In Wild Animal Medicine, ultrasonographic exams could be considered as a tool to diagnose and prevent disease. Ultrasonographic examination of twenty snakes (Boa constrictor) was performed in order to describe the morphological and ultrasonography appearance of their coelomic structures. Examination by ultrasonography revealed the parechymal liver ranging from slightly hypoechogenic to hyperechogenic, with echogenic margins and homogeneous echotexture in total scanning of this organ. The kidney was accessible for examination by sagittal ultrasound, showing an ellipsoid shape, a hyperechogenic capsule with a thin and regular wall. Ultrasonographic scanning of ovarian follicle showed an ovoid shape, as well as a thin, regular and slightly hyperechogenic edge. No evident structures from male reproductive system were identified by ultrasound examination because of their similar echogenic appearance to the tissues around and the "body fat" that lies in this region. Examination by ultrasound of the coelomic structures was a fast and repeatable technique, which provides useful data to recognize the morphology, sintopy and ultrasonographic appearance of organs such as liver, kidneys and vitellogenic follicles.INDEX TERMS: Boa constrictor snake, ultrasound, wild animals, Amazon.
Sperm cryopreservation is very important to genetic material quality dissemination. However, the spermatozoa can be damaged during this process. The objective of this study was to investigate the functionality of spermatozoa plasmatic membrane of ovine after cryopreservation. The work was conducted at Laboratory of Reproduction Biotechnology, Veterinary Medicine course, Federal University of Piaui. Fifteen animals were used (5 Dorper, 5 Santa Inês, and 5 animals of undefined breed - SRD). The animals were between 2 and 3 years old, and only one ejaculate was used per animal. Semen was collected via artificial vagina and frozen in cryopreservation machine (TK 3000®, OV curve 2), with Tris-yolk base extend and glycerol. The semen was evaluated before and after cryopreservation. To investigate the functionality of spermatozoa plasmatic membrane hypo-osmotic test (HOST) was used. After thawing, 20 μL of semen was incubated in 2 mL of hypoosmotic solution (125 mOsmol L-1) and 20 μL of semen was incubated in a 4% formalide citrate solution at 37°C for 1 h. After incubations, both samples were placed on microscopic slides, and 200 cells were visually evaluated per slide. Data were analyzed by Tukey-Kramer test (parametric data) and Dunn test (no parametric data) by Grafpad Instat® software. The motility averages after thawing were 36 ± 15.2% (Dorper), 36 ± 19.5% (Santa Inês), and 21 ± 14.3% (SRD). If the plasma membrane over the principal piece is intact, the membrane will swell, causing the tail to coil, whereas spermatozoa with damaged principal piece membranes will not swell. The HOST averages were 24.2 ± 11.8% (Dorper), 21% ± 6.4 (Santa Inês), and 10.4 ± 1.6% (SRD). Motility and HOST showed positive correlation (P < 0.001). The SRD animals showed higher correlation numbers than other groups (P < 0.009). The HOST is a cheap, easy, and efficient technique to estimate the functionality of sperm plasmatic membrane. We suggest that other studies could be done with more animals and more samples.
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