The present work sought to characterize the cyanogenic glycoside dhurrin in five sugarcane varieties and to determine its possible relationship with the formation of ethyl carbamate in cachaça. For each variety, methanol, ethyl acetate and hexane extracts were prepared and submitted to thin layer chromatography. Chromatographic spots were revealed with iodine vapors. The physical and chemical parameters of the spirits produced from the five different varieties of sugar cane and by different production processes were within the legal limits. Although not exceeding the limit for ethyl carbamate, a significant variation in the final concentration of this compound was observed for each sample analyzed. The R f values for the dhurrin standard and for the sugarcane variety SP 83-2847 were equal, similar to those for SP 80-3280 and CTC 11 and different from the R f values for the RB 86-7515 and IAC 86-2480 samples.
The coagulating potential of the crude extract from the barbatimão bark for the treatment of dairy effluent was assessed and compared with industrial coagulants such as aluminum sulfate (inorganic coagulant), the crude extract of black wattle bark (used in the manufacture of natural organic coagulants), and gallic acid (the main constituent of condensed tannins). The aqueous extract of barbatimão was obtained by refluxing for 12 h, and the black wattle was provided by a producer of natural coagulants. The determinations of total phenolic compounds and tannins in the extracts were performed by the Folin-Ciocalteu method and precipitation with casein, respectively. The treatment of the effluent with coagulant was performed by the Jar-Test. After testing, the effluent was evaluated for turbidity, total solids, and biochemical oxygen demand (BOD). A higher content of phenolic compounds (19.81% mg of gallic acid/g extract) was observed in the barbatimão extract than in the black wattle extract (14.24% mg of gallic acid/g extract). However, the extracts contained the same amount of tannins, 13.95% for the barbatimão extract and 12.89% for the black wattle extract. A 63%, reduction in BOD was observed for the effluent treated with barbatimão extract, 35% for the black wattle extract, 58% for gallic acid and 60.59% for the treatment with aluminum sulfate. Treatment with gallic acid caused a 98% reduction in the turbidity of the effluent; with aluminum sulfate, a 97% reduction was obtained
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