The development of innovative ingredients through biotechnological routes has established insect proteins as an emerging source of bioactive peptides. The current study aimed to evaluate the antioxidant properties of black cricket (Gryllus assimilis) protein hydrolysates produced using the proteases FlavourzymeTM 500L, AlcalaseTM 2.4L, and NeutraseTM 0.8L, either individually or in binary/ternary combinations. The enzymatic hydrolysis promoted an increase of approximately 160% in total antioxidant capacity and 93% in the ferric reducing antioxidant power. The isolated use of the enzyme FlavourzymeTM 500L showed the most prominent positive effect on the antioxidant properties, presenting an IC50 value of 455 and 71 µg/mL for DPPH and ABTS radicals scavenging activities, respectively. This sample was composed mainly of small peptides (MW < 3 kDa), in which the antioxidant properties increased after fractionation by ultrafiltration. Gel electrophoresis analysis showed protein hydrolysates composed mainly of polypeptide chains with a mass of less than 14 kDa. Finally, the enzymatic treatment proved to be an efficient process to improve the antioxidant properties of black cricket proteins, increasing the possibility of applying these hydrolysates as bioactive ingredients in food or nutraceutical products.Practical ApplicationInsects represent an alternative source of proteins. Their modification through hydrolysis allows for the acquisition of compounds with great potential in industrial applications, such as functional ingredients or for nutraceutical purposes. The use of our experimental design proved to be an adequate tool for defining the best process conditions required for increasing the attainment of biologically active compounds.
Resumo O consumo de insetos como uma fonte alternativa de proteínas é considerado uma tendência futura e uma estratégia viável, com potencial notório para garantia do fornecimento de alimentos em nível global. Os insetos são uma fonte não convencional de proteínas, seja para consumo humano direto ou indiretamente, como ingredientes em alimentos formulados. Além disso, estudos científicos têm demonstrado que a hidrólise enzimática destas proteínas resulta na produção de peptídeos com atividades biológicas de grande interesse, como atividade antioxidante, antidiabética, anti-hipertensiva e antimicrobiana. O uso desses peptídeos com fim nutracêutico pode substituir ou reduzir o uso de drogas sintéticas, as quais estão associadas a efeitos colaterais indesejáveis. O presente trabalho teve como objetivo abordar o uso de insetos na alimentação humana, destacando sua aplicação como substrato proteico na hidrólise enzimática para produção de peptídeos bioativos. As principais propriedades bioativas dos peptídeos foram relatadas.
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