Correct orientation of cell division is considered an important factor for the achievement of normal brain size, as mutations in genes that affect this process are among the leading causes of microcephaly. Abnormal spindle orientation is associated with reduction of the neuronal progenitor symmetric divisions, premature cell cycle exit, and reduced neurogenesis. This mechanism has been involved in microcephaly resulting from mutation of ASPM, the most frequently affected gene in autosomal recessive human primary microcephaly (MCPH), but it is presently unknown how ASPM regulates spindle orientation. In this report, we show that ASPM may control spindle positioning by interacting with citron kinase (CITK), a protein whose loss is also responsible for severe microcephaly in mammals. We show that the absence of CITK leads to abnormal spindle orientation in mammals and insects. In mouse cortical development, this phenotype correlates with increased production of basal progenitors. ASPM is required to recruit CITK at the spindle, and CITK overexpression rescues ASPM phenotype. ASPM and CITK affect the organization of astral microtubules (MT), and low doses of MT‐stabilizing drug revert the spindle orientation phenotype produced by their knockdown. Finally, CITK regulates both astral‐MT nucleation and stability. Our results provide a functional link between two established microcephaly proteins.
Understanding the developmental levels of fundamental movement skills has a critical role in the improvement of motor competence in childhood. In this respect, the use of Microsoft Kinect to assess vertical jumping skill and to predict developmental levels in 9- to 12-yr.-old children was evaluated. 41 boys and girls repeated the countermovement jump test three times. Vertical jumping skill levels were categorized using observational records, while kinematic and temporal parameters were estimated using a biomechanical model based on data acquired by the Kinect. Multivariate analysis of variance (MANOVA) and discriminant analysis verified that the height of the jump and the flight height predict the primary differences in jumping skill developmental levels, and the Kinect-based assessment discriminates these levels.
High-level of technical skills and tactical behaviour are key factors for an optimal performance in soccer games. About the most used and innovative training methods, the small-sided games (SSGs) seem to be a worthy and valid methodology to train simultaneously many skills by reproducing several conditions of a real match. Accordingly, the aim of this study was to conduct a systematic review of the available literature to lead an improved understanding in the usefulness of SSGs for improving technical skills in soccer. To ensure a rigorous and reliable approach, the review was performed according to PRISMA (Preferred Reporting Items for Systematic review and Meta-analyses) guidelines. 1031 records were initially identified, but only 26 studies were further included in the review. Moreover, other two studies were added after a check of the bibliography of two major reviews. So, each of these studies was analysed according to the aforementioned guidelines and using inclusion criteria related to SSG and training in soccer. The current analysis provided several indications and recommendations for coaches and trainers of soccer teams in order to improve several technical skills by means of the use of SSGs. Furthermore, several evidences for using SSGs in school environment are also provided.
The aim of this study was to identify the independent and interactive effects of possession strategy, pitch location, and game period on the offensive actions performed by the winning teams in the 2012 European Football Championship. The non-clinical magnitude-based inferences method was used to interpret the true effect of the performance indicators on the response variable. The offensive team possessions were grouped into winning (n = 2035) and losing (n = 2071). The winning teams performed offensive processes mainly using the possession play strategy (OR: 0.75, very likely negative effect of the direct play). When the analysis included the pitch location, negative interaction effect was found for the direct play, which ended up in the central path (OR: 0.70, very likely negative effect). On the contrary, the direct play in the second half of the match seemed to produce an effect on the probability of the winning teams performing offensive processes (OR: 1.59, most likely positive effect). The results of multivariate analyses showed that the offensive team possession profiles required a careful investigation because the possession strategy changed under the conjoint effect of pitch location and game period.
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