The occurrence of late blowing defects in cheese produces negative effects on the quality and commercial value of the product. In this work, we verified whether the addition of raw jenny milk to bulk cow milk reduced the late blowing defects in semihard cheeses. During cheesemaking, different aliquots of jenny milk were poured into 2 groups of 4 vats, each containing a fixed amount of cow milk. A group of cheeses was created by deliberately contaminating the 4 vats with approximately 3 log10 cfu/mL milk of Clostridium tyrobutyricum CLST01. The other 4 vats, which were not contaminated, were used for a second group of cheeses. After 120 d of ripening, some physical, chemical, and microbiological parameters were evaluated on the obtained semihard cheeses. Differences in sensory properties among cheeses belonging to the uncontaminated group were evaluated by 80 regular consumers of cheese. Our results showed that the increasing addition of jenny milk to cow milk led to a reduction of pH and total bacterial count in both cheese groups, as well as C. tyrobutyricum spores that either grew naturally or artificially inoculated. We observed a progressive reduction of the occurrence of late blowing defects in cheese as consequence of the increasing addition of jenny milk during cheese making. Moreover, the addition of jenny milk did not affect the acceptability of the product, as consumers found no difference among cheeses concerning sensorial aspects. In conclusion, the important antimicrobial activity of lysozyme contained in jenny milk has been confirmed in the current research. It is recommend for use as a possible and viable alternative to egg lysozyme for controlling late blowing defects in cheese.

The agriculture account is 92% of the global freshwater footprint, and 29% of this amount is used in animal husbandry to produce forage, to mix animal feed, for drinking of the animals and, at least, in the farm activities. In this trial we tested two diets whose production is characterized by different water consumption. Two homogeneous groups of milking cows were used to compare two different diets: standard feeding, with corn silage-based diet; and alternative feeding, with triticale silage-based diet. Both silages represented about 47% of diet composition. Diets were characterized by the same energy and protein content. Despite the lowest water consumption of the triticale silage group, no significant differences were observed between the groups on production level and on milk chemical composition.

In the present study, an original article about Podolian cattle and its milk was performed. In detail, the following factors on reproductive career of Podolian cattle in semi-extensive and extensive rearing were analysed: age of calving, gestation lenght, calving interval length, abortions percentage, conception and calving period. Chemical and phisycal parameters (protein, fat and lactose), somatic cell count, lisozyme content and antiradical activity, with ABTS and DPPH assays, of Podolian milk in semiextensive and extensive rearing were evaluated. Finally, suggestions on future prospects of Podolian milk use were proposed: the preparation of hand soap with 5 percentage of podolian milk and the potential role of this breed for prevention and propagation of fire. The data concerning the reproductive career, milk and envinromental role of 677 cows registered in the Herdbook were analysed. The results showed that the 58.56% of the cows manifested a calving interval of 11-14 months. The reproductive activity occurred in spring; over 70% of the cows calved in the period February-April. The chemical-physical aspects of milk are in agreement with literature. It presents an high antioxidant activity values for each group (97.03% and 97.50% for ABTS and 52.09% and 52.60% for DPPH, semiextensive and extensive system respectively). The consumer test shows that the subjects appreciated the soap containing 5% of Podolian milk for different aspect. The data about environmental role were obtained from Podolian cows reared in fifteen grazing areas were monitored for five years during the summer pasture, when the Podolian cattle graze on green grazing areas located in Basilicata region (South of Italy). Therefore, an efficient management of grazing by Podolian cattle could be an important tool to prevent the fire propagation.

The use of a sterilized product for washing cows' udders before milking may be useful to reduce or prevent Clostridium tyrobutyricum contamination, the main cause of the late-blowing defect in hard and semi-hard cheeses. The aim of this research was to evaluate the antibacterial efficacy of an experimental formula containing 15% condensed donkey milk (lysozyme content 825 mg/L). The antimicrobial activity of condensed milk was first evaluated in vitro, using the disk diffusion method, on the following microorganisms: Bacillus megaterium, Bacillus mojavensis, Clavibacter michiganensis, and Clostridium tyrobutyricum. These results were compared with the effects of 2 antibiotics, ampicillin (100 mg/mL) and kanamycin (50 mg/ mL), and a commercial pre-dipping formula. The results showed that the inhibitory activity of lysozyme from donkey milk on all the considered microorganisms was higher than that of the commercial product and similar to that of the 2 antibiotics. Next, the formula with lysozyme was compared with a commercial pre-dipping formula on 48 lactating cows (24 cows in each group). Skin tests were performed on teats before and after pre-dipping. Results showed that the formula with condensed milk significantly reduced the clostridial load detected on the skin of cows' teats before cleaning (−55.61% vs. −27.99%) and in the bulk milk of the experimental group compared with the control group with commercial product (−52.53% vs. −32.42%).

The aim of this study was the development of a model for evaluating dairy cow ration efficiency. This model took into account technical, metabolic, and economic parameters, which were divided into two main categories: input and output. Feeding (food administered and its nutritional characteristics) was considered as the input parameter. The output indicators were directly or indirectly correlated with feeding, and included: quality and quantity of milk, body condition score, live weight, reproductive parameters, incidence of animal diseases (laminitis), undigested fraction, fecal consistency, feed efficiency (FE), and income over feed cost (IOFC). The model was validated using ten dairy farms located in the northwest of Basilicata. The farms were divided into two groups (A and B) as a function of the urea level in bulk milk. In Group A, the urea level was between 25 and 31 mg/100 mL milk, whereas, in Group B, the range was 21-22.5 mg/100 mL milk. The model showed that the values of reproductive parameters were worse in Group A than in Group B. However, the Group A showed better milk qualitative and quantitative characteristics, such as a high average production per head (28.15 vs 26.93 kg), and a high fat (3.92 vs 3.71%) and protein (3.53 vs 3.37%) content of bulk milk. Moreover, the highest values of FE (1.45 vs 1.35 kg milk/kg dry matter) and IOFC (6.07 vs 5.32 €) were found in Group A. The model clearly showed that the administration of unbalanced rations, based on the physiological stage of the animals, negatively affected both the qualitative and quantitative characteristics of milk, as well as the reproductive performances. The administration of unbalanced rations for the energy/protein content caused dysmetabolic syndromes, which led to a reduction of both FE and IOFC. This, ultimately, caused a fall in the overall farm profitability.