The interest in the welfare of zoo animals, from both the public and the scientific community, has long been biased towards mammals. However, growing evidence of the complex behavioural repertoires of less charismatic animals, such as reptiles, reveals the necessity to better comply with their welfare needs in captivity. Here, we present the effects of an enclosure change towards a more natural habitat in captive Galapagos tortoises (Chelonoidis spp.) held at ZSL London Zoo. Using behavioural observations, we found that the tortoises habituated to their new enclosure in six days. This represents the first quantification of habituation latency to a new enclosure in a reptile model to our knowledge—which is important information to adapt policies governing animal moves. The tortoises expressed time budgets more similar to those of wild individuals after their transition to the new enclosure. Interestingly, the hierarchy between the individuals was inverted and more stable after this change in environment. The tortoises interacted less often, which led to a decrease in the frequency of agonistic encounters. We also found that higher ambient sound volume was associated with increased likelihood of interactions turning into fights. Taken together, our results demonstrate the potential of appropriate enclosure design to improve reptile welfare.

Maintaining Gymnophiona in captivity provides opportunities to study the behaviour and life-history of this poorly known Order, and to investigate and provide species-appropriate welfare guidelines, which are currently lacking. This study focuses on the terrestrial caecilian Herpele squalostoma to investigate its sensitivity to disturbances associated with routine husbandry needed for monitoring and maintaining adequate wellbeing in captivity. Fossorial caecilians gradually pollute their environment in captivity with waste products, and substrate must be replaced at intervals; doing so disturbs the animals directly and via destruction of burrow networks. As inappetence is frequently associated with stress in amphibians, the percentage consumption of offered food types, river shrimp (Palaemon varians) and brown crickets (Gryllus assimilis), was measured as an indicator of putative stress following three routine substrate changes up to 297 days post-substrate change. Mean daily variation in substrate temperatures were also recorded in order to account for environmental influences on food consumption, along with nitrogenous waste in tank substrate prior to a substrate change and fresh top soil in order to understand the trade-off between dealing with waste accumulation and disturbing animals. We found a significant negative effect of substrate disturbance on food intake, but no significant effect of prey type. Variations in daily soil temperatures did not have a significant effect on food intake, but mean substrate temperature did. Additionally, substrate nitrogenous waste testing indicated little difference between fresh and tank substrate. In conclusion, this study provides a basis from which to develop further welfare assessment for this and other rarely kept and rarely observed terrestrial caecilian species.

Mountain chicken frogs Leptodactylus fallax are assessed as Critically Endangered on the IUCN Red List due to threats including chytridiomycosis and habitat loss. Ex-situ populations underpin species survival, but captive management is hampered by incomplete species knowledge, including its behavioural biology. In sixteen adult frogs, we investigated enclosure usage, nocturnal activity budgets, and behavioural responses to varying levels of husbandry-related disturbance through instantaneous scan sampling of camera trap footage over forty-two consecutive nights. Enclosure usage was quantified through the application of modified Spread of Participation Indices (mSPI). We present the first detailed activity budgets published for this species and found that broad activity patterns of captive animals corresponded well with their wild conspecifics; taking refuge during the day and emerging at dusk into exposed areas of the enclosure. Some behaviours, especially hunting and bathing, were partially phased throughout the nocturnal period. Enclosure use was not even, with disproportionately large amounts of time spent using nest boxes and deep leaf litter, even at night, and the converse in thin leaf litter and elevated perches. Principal Components Analysis (PCA) identified a bold-shy behavioural grouping (movement and resting opposed to refuge behaviours) that was consistent across husbandry-related disturbance conditions. Randomisation analyses showed that disturbance significantly affected multiple measured behaviour, especially feeding, social interactions and vocalisation; the impact was associated with the degree of disturbance. Novel behaviours were also documented, which are thought to be related to courtship. Our data provide insights into the behaviour of mountain chicken frogs and highlight potential trade-offs between enclosure maintenance, veterinary intervention, and animal welfare. Keywords: Amphibians, behaviour, mSPI, disturbance, welfare, Zoo

Multiple clutching, with two or three successful clutches raised in a single breeding season, is reported from two females of Leptodactylus fallax in two European zoos. Previously, only single clutches were known to be raised by this species. Multiple clutching is perhaps unexpected in this species due to its resource-heavy parental care behaviour. Potential drivers of multiple clutching, including food resourcing and timing and size of initial clutches, are discussed.

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