Many behavioral traits and most brain disorders are common to males and females but are more evident in one sex than the other. The control of these subtle sex-linked biases is largely unstudied and has been presumed to mirror that of the highly dimorphic reproductive nuclei. Sexual dimorphism in the reproductive tract is a product of Mü llerian inhibiting substance (MIS), as well as the sex steroids. Males with a genetic deficiency in MIS signaling are sexually males, leading to the presumption that MIS is not a neural regulator. We challenge this presumption by reporting that most immature neurons in mice express the MIS-specific receptor (MISRII) and that male Mis ؊/؊ and Misrii ؊/؊ mice exhibit subtle feminization of their spinal motor neurons and of their exploratory behavior. Consequently, MIS may be a broad regulator of the subtle sex-linked biases in the nervous system.anti-Mü llerian hormone ͉ exploratory behavior ͉ motor neuron ͉ sexual dimorphism
Mesenchymal stem cells (MSCs) form part of tumor stroma, and are typically considered to be pro-tumorigenic, promoting continuing tumor growth and metastasis. Here, we describe a mechanism by which MSCs may be anti-tumorigenic, through inhibition of breast cancer cell migration and invasion, an important part of metastasis. MDA-MB-231 and T47D cells were co-cultured in a Transwell insert above MSCs or MSC conditioned media and their migration or invasion through Matrigel measured. The conditioned media was found to inhibit breast cancer cell movement. TIMP-1 and TIMP-2, inhibitors of matrix metalloproteinases (MMPs), were identified as candidates for this inhibition and enhanced secretion of MMPs was not sufficient to counter the anti-migratory effects of TIMP expression. Inhibition of TIMP activity showed that TIMP-1 and TIMP-2 together were largely responsible for the reduction of migration and invasion by MSCs. Therefore, MSCs may play anti-tumorigenic, anti-metastatic roles in tumor development, with the overall outcome dependent upon the balance of pro-and anti-tumorigenic molecules secreted.
Edited by Laszlo NagyKeywords: Amhr2 Dominant-negative Kinase domain Ligand binding domain Bone-morphogenetic protein responsive element a b s t r a c t Anti-Müllerian hormone (AMH) has both paracrine and hormonal actions that occur at different AMH concentrations, and in cells with different densities of its specific receptor (Amhr2). This diversity is not explained by canonical AMH signaling. We report that Amhr2 has two splice variants: Amhr2D2 (AMH binding site) and Amhr2D9/10 (kinase domain). Both spliced variants inhibit AMH signaling in a reporter assay. The mRNA for the spliced variants was relatively less abundant than Amhr2 mRNA in all tissues. This suggests that the physiological function(s) of the receptor variants may be restricted to specific cellular/subcellular sites and/or to the transport of AMH.
Pinnatoxin G is a cyclic imine neurotoxin
produced by dinoflagellates
that has been reported in shellfish. Like other members of the pinnatoxin
family, it has been shown to have its effects via antagonism of the
nicotinic acetylcholine receptors, with preferential binding to the
α7 subunit often upregulated in cancer. Because increased activity
of α7 nicotinic acetylcholine receptors contributes to increased
growth and resistance to apoptosis, the effect of pinnatoxin G on
cancer cell viability was tested. In a panel of six cancer cell lines,
all cell types lost viability, but HT29 colon cancer and LN18 and
U373 glioma cell lines were more sensitive than MDA-MB-231 breast
cancer cells, PC3 prostate cancer cells, and U87 glioma cells, correlating
with expression levels of α7, α4, and α9 nicotinic
acetylcholine receptors. Some loss of cell viability could be attributed
to cell cycle arrest, but significant levels of classical apoptosis
were found, characterized by caspase activity, phosphatidylserine
exposure, mitochondrial membrane permeability, and fragmented DNA.
Intracellular Ca2+ levels also dropped immediately upon
pinnatoxin G treatment, which may relate to antagonism of nicotinic
acetylcholine receptor-mediated Ca2+ inflow. In conclusion,
pinnatoxin G can decrease cancer cell viability, with both cytostatic
and cytotoxic effects.
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