Human activities like urbanisation, the replacement of traditional agriculture systems by modern industrial methods or the introduction of modern high-yielding varieties may pose a danger to the biological diversity. Using microsatellite markers, we analysed samples of cultivated wheat ( Triticum aestivum L.) collected over an interval of 40-50 years in four comparable geographical regions of Europe and Asia. No significant differences in both the total number of alleles per locus and in the PIC values were detected when the material collected in the repeated collection missions in all four regions were compared. About two-thirds of the alleles were common to both collection periods, while one-third represented collection mission-specific alleles. These findings demonstrate that an allele flow took place during the adaptation of traditional agriculture to modern systems, whereas the level of genetic diversity was not significantly influenced.

Food and nutritional insecurity constitute a main challenge in most Sub-Sahara African countries. Efforts to provide diets with sufficient nutrients such as proteins, carbohydrates, vitamins and essential minerals should include the introduction of new vegetable and legume crops. Vegetable soybean "edamame", is a nutritious vegetable legume well known and consumed in Asia and America, but underutilized in Africa. This review paper aims at documenting the existing information on edamame and analyzing the potentials for its use in Sub-Saharan Africa. The analysis of the existing literature revealed that vegetable soybean provides great advantages in term of production because of good market value of the fresh pods having a good market value and high demand on both local and international markets. Then, the consumption of edamame can also really contribute to reducing nutritional deficiencies in children and even adults, through its great nutritional content and good health benefits. Therefore, edamame is a good crop to promote in Africa. The promotion of edamame requires many research activities starting from evaluation of agro-ecological adaptation, determination of consumers' preferences and genetic improvement based on farmers, processors and consumers' needs, in order to sustain a seed system for the crop.

Knowledge of genetic diversity is essential for any future varietal improvement program. Thus, the objective of this study is to contribute to a better knowledge of the genetic diversity within 15 sesame accessions, using biochemical markers and RAPD. The accessions are of various sources and seeds of different colors; color being an important feature in the sesame market. SDS-PAGE on protein extracts of sesame seeds revealed 31 protein markers, only 4 of which were polymorphic, indicating that this technique is not suitable for studying genetic diversity in S. indicum. The analyses on isoenzymatic extracts of esterase from sesame seedlings made it possible, to distinguish and classify the accessions into 2 major groups. RAPD analysis of genomic DNA using 10-mer primers revealed a high inter-accession genetic diversity. A total of 53 DNA markers, including 48 polymorphic, were produced. The coefficient of similarity between accessions ranged from 0.343 to 0.897. RAPD markers were found to be more suitable for studying genetic diversity within sesame germplasm than isozyme analyzes. Nevertheless, geographical distributions and seed colors are not related to the classes formed.

Background: African bush mango tree is an important fruit plant with high nutritional, medicinal, and commercial values. However, its seedling system remained a deep understanding. This study aimed to evaluate the effect of topophysis and growth regulators on the reactivity of different types of buds and zygotic embryos of wild mango. Methodology: Ripe fruits from two local varieties (Wossro and Sissro) were pulped. The nuts were extracted and dried for one week under greenhouse. Zygotic embryos were excised from nuts and disinfected with the bleach solution (NaClO 10% with 8% active chloride) for 10 min following by three successive rinsing using sterilized water. The second set of nuts was cultivated for under greenhouse in the polybags containing the sand. One month later, buds from different positions (apical, axillary and cotyledonary) were excised and disinfected with NaClO 10% for 10 min follow by the immersion in a mercuric chloride 0.1% added with two drops of Tween 20 especially for axillary and cotyledonary buds for 5 min while 0.01% of mercuric chloride was used for apical buds. The explants were cultured on ¼MS and ½WPM media supplemented with BAP, KIN at different concentrations (0.2 mg/L and 3.5 mg/L respectively) and NAA (0.05 mg/L). Results: The best sprouting rate was obtained with the variety Wossro which showed a high bud break rate (26.47%) against (9.88%) for the variety Sissro. The topophysis significantly influenced the response of the buds in tissue culture. 24.48% of axillary buds were sprouted on ¼ MS medium + 3.5 mg/L BAP+ 0.05mg/L NAA. The in vitro germination of embryos was significantly (p≤ 0.05) influenced by the genotype of the variety.  50.76% of buds were germinated for Wossro while 18.32% were germinated for Sissro. ¼ MS + 0.2 mg/L KIN has significant influenced the plants growth and development. Implication: The findings will help to improve the regeneration rate and plantlets production of African bush mango tree.