Extraction of RNA from eukaryotic cells is a crucial stage in many molecular biology studies. Thus it is essential to use the most appropriate method that maximizes the yield and maintains the integrity of extracted RNA. The present work compared five manual RNA extraction methods of different dilutions of rabbit blood. The assessment of the concentration and purity of the extracted RNA were used as parameters to evaluate the efficiency of each method. Statistical analysis showed that the most effective method for RNA extraction from rabbit blood is by following the protocol of the RNeasy Lipid Tissue kit, but it requires the use of toxic reagents. The most efficient kit using no toxic reagents is the RNeasy protect animal blood.