International audienceBackground/purpose: Bisphosphonates (BPs) are synthetic drugs with antitumorand bone antiresorptive activities. The use of BPs is suspected to favor the emergence of osteonecrosisof the jaw (ONJ), a putative side effect whose pathogenesis remains unclear. Thus,we aimed to get insights about the impact of BPs on osteoblasts functions, using a threedimensional(3D) culture model, which is suggested to be more similar to the in vivo tissuesthan monolayers.Materials and methods: Effects of low (0.1 mM) and high (10 mM) concentrations of zoledronicacid were investigated on osteoblasts (hFOB 1.19), cultured as multicellular spheroids (MCS).Proliferation, apoptosis, spheroid growth kinetics, and morphology were studied using the 3-(4,5-dimethyl-thiazoyl-2yl) 2,5-diphenyl-tetrazolium bromide (MTT) and acid phosphatase(APH) assays, caspase 3 Western-blotting, phase contrast imaging and scanning electron microscopy(SEM).Results: Proliferation, apoptosis, and spheroid morphology showed that 10 mM zoledronic acid(ZA) induced a significant reduction in the relative viable cell number, correlated with morphologicalalterations of spheroids, and induction of apoptosis. A lower ZA concentration (0.1 mM)promoted cell proliferation without affecting growth kinetics or spheroid morphology.Conclusion: ZA sensitivity of osteoblasts depends on concentration and experimental models.The dual dose-dependent effects of ZA on osteoblasts cultured as spheroids, thereby promotingor inhibiting cell proliferation, may provide opportunities in tissue engineering. At last, thehFOB spheroid culture system represents a valuable model for the exploration of the molecula
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.