The aim of this study was to evaluate the use of endometrial cytology and metabolic profiles for selection of donor cows in embryo transfer programmes. For this purpose, 69 clinically healthy Holstein cows were enrolled in the study. At the start of the superovulation procedure (Day 0), blood and endometrial samples were obtained to determine metabolic and uterine status, respectively. The cows were then subjected to porcine follicle stimulating hormone (pFSH) superovulation treatment, and embryos were recovered after 7 days. The mean number of embryos obtained per flush was 9.89±8.21 (4.63±5.34 viable embryos, 0.82±2.01 degenerated embryos and 4.57±6.44 unfertilized ova). The following statistically significant variables were entered in a regression model: beta-hydroxybutyrate, serum cholesterol, body condition, number of calvings and percentage of neutrophils. In almost all cases, the model explained some percentage of the variance: total number of embryos, 4.8% (p<0.05); number of degenerate embryos, 4.2% (p=0.051); and number of unfertilized ova, 14.2% (p<0.01). Statistical models for the percentage of viable embryos and unfertilized ova accounted for 24.0% and 29.4% of the variance, respectively, and both were statistically significant (p<0.01). The model for the percentage of degenerated embryos was statistically significant (p<0.05) and explained 4.4% of the variance. In conclusion, we have demonstrated that positive energy balance and healthy uterus can improve ovarian response and the proportion of viable embryos in cows. Efficient tools for monitoring the metabolic and uterine status should therefore be used in bovine embryo transfer programmes.

No abstract

The aim of this study was to assess the effect of cooling on sperm motility before and after frozenthawed stallion semen. Fifteen ejaculates of three stallions were collected with artificial vagina. The progressive motility was determined under microscope immediately after collection, cooling (5°C for 0, 2, 7 or 24 h) before frozen-thawed and cooling (5°C for 0, 2, 7 or 24 h) after the semen was frozenthawed. Sperm progressive motility (83.1, 78.7, 74.8 or 70.3%, respectively) was significantly different (P<0.05) at different hours of cooling before freezing. Similar pattern was found when semen was subjected to cooling, frozen-thawed and cooling time resulted in a progressive reduction in motility from 39.4 to 26.9%. The motility of semen subjected only to cooling for 24 h before freezing was optimal (70.0%) for artificial insemination. Moreover, semen subjected to cooling for 7 or 24 h before and after frozen-thawed could be used still with some considerations for artificial insemination.