A novel bioactive depsipeptide, thiocoraline, was isolated from the mycelial cake of a marine actinomycete strain L-1 3-ACM2-092.Based on morphological, cultural, physiological, and chemical characteristics, strain L-13-ACM2-092was ascribed to the genus Micromonospora. Thiocoraline showed a potent cytotoxic activity against P-388, A-549 and MEL-28cell lines, and also a strong antimicrobial activity against Gram-positive microorganisms. This compoundbinds to supercoiled DNAand inhibits RNAsynthesis.
A novel bioactive macrolide, IB-96212 has been isolated from the fermentation broth of a marine actinomycete, L-25-ES25-008. The strain belongs to the genus Micromonospora. The macrolide showed a very strong cytotoxic activity against P-388, and lower but significant activity against A-549, HT-29, and MEL-28 cell lines. We describe the isolation, taxonomy and fermentation of the producing strain as well as the isolation ofIB-96212.A marine Micromonospora has been isolated from homogenates of a sponge. The strain has been found to produce a fermentation broth with cytotoxic activity. A new macrolide responsible for this activity has been isolated from this fermentation broth. Wedescribe in this paper the taxonomical characterization of the strain, its fermentation and the isolation of the active compound, together with the study of the in vitro activities. The structural elucidation is presented in an accompanyingpaper1}.
Materials and Methods
Mi cro organi sms
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