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The aims of this experiment were to evaluate the addition of coconut water in natura to the freezing media, compare the effect of deionized water vs filtered water of coconut over the post-thaw seminal characteristics, and evaluate the effect of the deionized water and in natura coconut water on the seminal characteristics of boar sperm at different post-thaw times. Thirty-four ejaculates were used divided in three aliquots which received one of the following treatments (T): T1, LEY (bidistilled water, lactose, and egg yolk) and LEYGO (LEY + glycerol and Orvus ET paste); T2, LEY(A) (coconut deionized water, lactose, and egg yolk)-LEYGO(A); and T3, LEY(B) (in natura coconut water, lactose, and egg yolk)-LEYGO(B). Samples of boar semen were frozen according to the Westendorf method, thawed at 38°C, and evaluated at three incubation times (0, 30, and 60 min). Seminal characteristics assessed were motility (Mot), acrosomal integrity (AInt), membrane integrity (MInt), and mitochondrial activity (MAct). T1 showed a higher percentage of viable sperm than T3 (Mot 36.5 vs 5.4 %, AInt 61.8 vs 41.2 %, MInt 50.4 vs 41.3 %, and MAct 56.9 vs 50.5 %). T2 kept a higher percentage of viable sperm at all incubation times. In natura coconut water showed a detrimental effect over the viability of the frozen-thawed boar semen. Deionized coconut water improved the boar semen viability post-thaw, outperforming results of in natura coconut water.

RESUMENLos objetivos del presente estudio fueron evaluar la libido y la capacidad de servicio de carneros de pelo adultos Pelibuey (PB), Blackbelly (BB), Dorper (DR) y Katahdin (KA) durante las tres épocas del año en el trópico. Dos veces por época 28 carneros maduros fueron individualmente expuestos durante 10 minutos a una oveja inmovilizada e inducida al estro. Se registró el número total de las actividades de la conducta sexual, el tiempo de reacción al primer servicio, y período de latencia entre servicios. El efecto de raza influyó en el tiempo de reacción al primer servicio y los tiempos de latencia (P < 0,05), excepto el tiempo de latencia en el segundo servicio, también la época resultó significativa (P < 0,05), pero no la interacción entre raza*época (P > 0,05). Todos los carneros realizaron al menos un servicio. La media (± EE) del tiempo de reacción y las latencias entre el segundo y tercer servicio fueron 37,62 ± 4,97; 136,43 ± 9,75 y 180,95 ± 11,87 segundos, respectivamente. Los carneros PB, BB y KA fueron más rápidos que los DR en el tiempo de reacción y latencia al segundo servicio (P < 0,05). Para la latencia al tercer servicio los carneros PB y KA fueron más rápidos que BB y DR (P < 0,05). Las actividades de la libido más frecuentes fueron el olfateo anogenital y el pateo de extremidades. Se concluye que bajo condiciones de clima tropical los carneros adultos PB, KA y BB mostraron mayor capacidad de servicio que los DR, con menor tiempo de reacción y latencia al segundo servicio. Los carneros de pelo exhibieron con mayor frecuencia actividades de olfateo anogenital y pateo de extremidades.Palabras clave: libido, capacidad de servicio, carneros de pelo, trópico. SUMMARYThe objectives of the present study were to evaluate the libido and serving capacity of Pelibuey (PB), Blackbelly (BB), Dorper (DR) and Katahdin (KA) hair sheep rams in three seasons under tropical environmental conditions. Twenty-eight mature rams were individually exposed for 10 minutes to an immobilized, oestrous-induced ewe twice per season. The total number of sexual behaviours, reaction time to first mating and latencies between mating were recorded. Reaction time and latencies were affected by breed effect (P < 0.05) and except for the latency time for the second ejaculation, the season was also significant (P < 0.05) while no interaction effects were observed (P > 0.05). All rams attained at least one ejaculation during testing. The overall mean (± S.E.M.) of reaction time and latencies to second and third mating were 37.62 ± 4.97s, 136.43 ± 9.75 and 180.95 ± 11.87 seconds, respectively. PB, BB and KA rams were faster than DR in reaction time and latency to second mating (P < 0.05). For latency to third mating, PB and KA rams were faster than BB and DR (P < 0.05). The libido activities that rams performed most frequently were anogenital sniffing and foreleg kicks. It was concluded that under tropical environmental conditions PB, KA and BB rams showed better serving capacity than DR, with shorter reaction time and latency t...

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