Hummingbirds possess a number of unique features. A recent report in Current Biology[1] showed that the Black Jacobin (Florisuga fusca), a Brazilian hummingbird in the Topazes clade, produces high-frequency vocalizations outside the known hearing range of birds. Here, we report that four hummingbird species in the Andean clade [2,3] also exhibit high-frequency vocalizations: the Ecuadorian Hillstar (Oreotrochilus chimborazo), with the highest fundamental frequency (mean F0 = 13.4 kHz), the Buff-tailed Coronet (Boissonneaua flavescens), Speckled Hummingbird (Adelomyia melanogenys) and Violet-tailed Sylph (Aglaiocercus coelestis). The presence of high-frequency vocalizations in hummingbirds belonging to different lineages poses the question of whether high-frequency vocalizations in this group of birds have been, so far, overlooked. These Andean species are closely related but live in two different habitats. Our characterization of ambient noise in each habitat suggests that the hummingbirds are exposed to different acoustic challenges, and that the frequency content of the vocalizations of the cloud-forest species is adapted accordingly. The function of these signals and the selection pressures driving their evolution remain unknown.

Some hummingbirds produce unique high-frequency vocalizations. It remains unknown whether these hummingbirds can hear these sounds, which are produced at frequencies beyond the range at which most birds can hear. Here, we show behavioral and neural evidence of high-frequency hearing in a hummingbird, the Ecuadorian Hillstar (Oreotrochilus chimborazo). In the field, hummingbirds responded to playback of high-frequency song with changes in body posture and approaching behavior. We assessed neural activation by inducing ZENK expression in the brain auditory areas in response to the high-frequency song. We found higher ZENK expression in the auditory regions of hummingbirds exposed to the high-frequency song compared to controls, while no difference was observed in the hippocampus between groups. The behavioral and neural responses show that this hummingbird can hear sounds at high frequencies. This is the first evidence of the use of high-frequency vocalizations and high-frequency hearing in conspecific communication in a bird.

Hummingbirds exhibit complex vocal repertoires that they use in their social interactions. Furthermore, they are capable of vocal production learning, an ability they share with songbirds, parrots, some non-oscine birds, and some mammals including humans. Despite these characteristics, hummingbirds have not received the same attention as other birds, especially songbirds and parrots in the study of vocal communication. Recent studies are advancing our knowledge of vocal communication in hummingbirds showing that these birds exhibit complex social learning and extraordinary abilities for vocal production. Moreover, vocal production learning in hummingbirds provides opportunities to study the evolution and diversification of vocal signals because of the presence of dialects in some species. In addition, the presence of high-frequency vocalizations in some hummingbirds underscores the relevance of these birds to study the evolution of communication signals and sensory adaptations. Not only do some species vocalize at unusually high frequencies compared to other birds, but evidence shows that at least one hummingbird species can hear these sounds, defying what we knew about avian hearing capabilities. Detailed descriptions of the hummingbird syrinx have shown that this organ exhibits homologous structures to those found in the syrinx of oscines, showing that vocal complexity in hummingbirds requires complex syringeal musculature. However, more research is needed to determine whether hummingbirds have unique adaptations that confer exceptional vocal and hearing abilities that exceed those found in other groups of birds.

Some species of Andean hummingbirds produce high-frequency vocalizations which exceed the vocal range of most birds. They also challenge our understanding of the role of habitat structure in the evolution of vocal signals because these hummingbirds live in strikingly different habitats, ranging from cloud forest to high-altitude grasslands. Although these vocalizations are produced at high frequencies, they exhibit considerable variation in frequency content and temporal structure. The calls of the hummingbirds from the cloud forest are simpler and have a narrow frequency range compared to the complex song of the grasslands hummingbird. We hypothesized that each of the three high-frequency vocalizations is adapted for transmission in their habitat. We characterized the transmission of high-frequency vocal signals in the cloud forest and in the grasslands. All vocalizations attenuated and degraded substantially at short distances, suggesting that they are adapted for short-range communication. The simple vocalizations of the cloud-forest species transmitted better in both environments compared to the complex song of the grasslands hummingbird, probably due to relaxed constraints for high-frequency sounds in open habitats.