Basil (Ocimum basilicum L.) is one of the main condiments for fresh consumption and essential oil production. The aim of the present work was to assess the agronomic characterization and analyze the essential oil of 63 basil accessions. The experiment was conducted in two stages in a greenhouse using vases and a hydroponic cultivation system. Oil extraction was performed employing the Soxhlet method. There was a significant variation in the agronomic characteristics among the evaluated accessions. The estimated total contents of essential oils ranged from 0.05 to 0.40%, and the major volatile fraction was methyl-eugenol. In the present study, accessions with superior performance compared to commercial varieties were found. Accession BL11 presented agronomic characteristics suitable for cultivation in a hydroponic system due to its better plant structure and late flowering. Accession BL24 stood out for essential oil extraction, producing 17.6% of linalool and a high intensity of color and odor. Accessions BL11 and BL24 presented market potential, given their higher mass productivity and higher essential oil yield, respectively. These accessions can be made available as new varieties in addition to being used in genetic improvement programs for this species.
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