BACKGROUND: Teucrium polium L. aerial parts are traditionally used in Algerian folk medicine due to its therapeutic properties. OBJECTIVE: The present study was designed to evaluate the effect of different extracting solvents (methanol, ethanol, ethyl acetate, chloroform, hexane and water) on antioxidant capacity and total phenol and flavonoid contents of Algerian Teucrium polium aerial parts. METHODS: The antioxidant capacity of Teucrium polium extracts was assessed by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) free radicals scavenging and reducing power assays. For each extract, the total phenol and flavonoid contents were investigated by the Folin-Ciocalteu and aluminium trichloride methods, respectively. RESULTS: All the tested extracts showed an appreciable total phenolic and total flavonoid contents and strong antioxidant capacity. Among the different extracts, the methanolic extract was found to be containing the highest amount of total phenols (206.95±1.82 mg of gallic acid equivalent per gram of extract) and flavonoids (42.16±0.61 mg of quercetin equivalent per gram of extract). The same extract also exhibited a much better reducing power and scavenging capacity against DPPH (IC50 = 6.77±0.15 mg/l) and ABTS (IC50 = 2.79±0.07 mg/l). CONCLUSION: These findings suggest that aerial parts of Teucrium polium methanolic extract could be used as natural source of antioxidants in food, pharmaceutical and cosmetic industry.
There is an important interest to research new protein sources. In this context, Microalgae, particularly Spirulina (Arthrospira platensis), seems to be a good alternative due to its wealthy nutritional composition. The aim of this study is to optimize Spirulina functional properties and to extract protein from whole biomass using (RSM). Ultrasound wad used as pre-treatment to optimize functional properties and to extract proteins from Spirulina powder by isoelectric precipitation. The effect of ultrasound and remaining parameters (pH, temperature, solid to liquid ratio and time) was evaluated by Box-Behnken design. The model was fitted by ANOVA analysis. ANOVA analysis showed a significant model (p < 0.05) for functional properties and protein extraction. The protein content of Spirulina powder was found to be 55% (w/w). There is a significant effect of ultrasound on functional properties and protein extraction from Spirulina. The optimum Water Holding Capacity (WHC) was 4.97 g H2O/g Spirulina powder, obtained at pH 4, 50 W power and 5 min sonication. The optimum Oil Holding Capacity (OHC) is 2.3 g H2O/g Spirulina powder, it was obtained at 50°C temperature, 70 w power and 10 min sonication. Ultrasound has a significant effect on functional properties and protein extraction from Spirulina. Arthrospira platensis grown in Algeria could be incorporated in foodstuff as natural supplement to improve nutritional value and consumer acceptability.
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