Somatic chromosomes of two Scorzonera species from subgenus Scorzonera, section Intricatae collected from four localities in southeast Iran were observed. In S. tortuosissima, 2n=28 was observed. S. intricata revealed two chromosome numbers of 2n=20 and 21. The chromosome numbers of 2n=21 had one B chromosome then the basic number is x=10 which is a new basic chromosome number for Scorzonera. Both species showed a predominance of metacentric chromosomes in each karyotype. The karyotype was formulated as 12m+2sm for S. tortuosissima and 8m+2sm for S. intricata.
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