Parkinson’s disease (PD) is characterized as bradykinesia and sleep disorder, troubling numerous people. In the present study, we aimed to explore whether reactive oxygen species (ROS)/caspase 3 promotes PD to provide a basis for novel treatments of PD. Firstly, we applied 1-methyl-4-phenylpyridinium
(MPP+) to stimulate PC12 cell lines to establish a PD cell model. Western blot and qRT-PCR analyses detected protein and mRNA expression of caspase 3, IL-1β, Bax, and BCL2. Finally, ROS detection kit determined ROS content. Compared with the controls, MPP+-treated PC12 exhibited
significantly elevated caspase 3, caspase 3, and IL-1β at the protein level (p < 0.001). In addition, MMP + treatment increased Bax protein level in vitro, while decreased Bcl-2 protein expression (p <0.001). Moreover, MPP + induced oxidative stress which
contributes to autophagy. The ROS in MPP + group was increased significantly (p < 0.001). ROS and caspase 3 participate in the pathogenesis of PD and enhances autophagy of nigral dopaminergic neuron.
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