No abstract

A hydrostable 2D Zn-based MOF, {[Zn(5-PIA)(imbm)]·2H2O}n (1) (5-H2PIA = 5-propoxy-isophthalic acid, imbm = 1,4-di(1H-imidazol-1-yl)benzene), was synthesized and structurally characterized. Complex 1 shows good water and thermal stability based on the TGA and PXRD analyses and displays a 2D framework with 1D channels of 4.8 × 13.8 and 10.0 × 8.3 Å2 along the a axis. The 5-fluorouracil (5-FU) payload in activated complex 1 (complex 1a) is 19.3 wt%, and the cumulative release value of 5-FU at 120 h was about 70.04% in PBS (pH 7.4) at 310 K. In vitro MTT assays did not reveal any cytotoxic effect of NIH-3T3 and HEK-293 cells when the concentration of 1 was below 500 μg/mL and 5 μg/mL, respectively. No morphological abnormalities were observed on zebrafish exposed to complex 1.

No abstract

Skeletal muscle is rich in lymphatic vessels, with an abundant blood supply, and it is an infrequent site of cancer metastasis. Previous studies have demonstrated that enhanced secretion of MyoD may occur when skeletal muscle is injured or becomes cancerous. It was hypothesized that MyoD may act as an endogenous cytokine to inhibit the proliferation of cancer cells. To verify the possible effect of this protein on tumor cell proliferation, C2C12 mouse skeletal muscle cells and 4T1 mouse breast cancer cells were co-cultured using embedded Transwell plates. Following co-culture, cell cycle analysis revealed that C2C12 muscle cells were able to inhibit the proliferation of the breast cancer cells. Subsequently, MyoD was silenced in C2C12 cells to assess its effect on 4T1 cell proliferation. Following co-culture with MyoD-silenced cells, a 5-ethynyl-20-deoxyuridine assay indicated that MyoD silencing prevented the reduction in proliferation of 4T1 cells induced by untransfected C2C12 cells. In summary, the results indicated that MyoD inhibits the proliferation of breast cancer cells and may be a tumor suppressor factor.

Online teaching has become more prevalent in recent years as a result of the process of digitization and adaptation to the times. Classroom instruction is no longer just conducted in person offline. Online education has received a lot of promotion, especially in the wake of the epidemic’s effects, and has evolved into a blended teaching model that combines the two modes. The old system of measuring the effectiveness of teaching is no longer reliable or scientific in assessing the new blended teaching model, and it has many flaws. A perfect system for evaluating the effects of blended learning must therefore be built. In order to more accurately assess the teaching impact of the blended teaching model, this study aims to build a more comprehensive system for evaluating blended teaching effects. This paper introduces the Kirkpatrick four-level evaluation model, which plays a significant role in the evaluation field, in order to achieve this goal. The experiments in this study compared the Kirkpatrick model with the AHP, which is frequently used in the evaluation. Online, offline, and blended teaching models were the subject of an experimental investigation. The experimental results demonstrated that each index combination’s weights in the Kirkpatrick model and the AHP model of the blended teaching effect evaluation system were fairly close to one another. The evaluation indicators all center on the assessment of practical ability after class, which is consistent with their focus. The innovation evaluation of course papers has the highest weight, and the weight of evaluation indicators is greater than 0.15; the Kirkpatrick model has the lowest weight of the number of platform logins, with a weight value of 0.011, and the evaluation index of the teaching effect evaluation system based on AHP.