Cowpea (Vigna unguiculata) and mung bean (Vigna radiata) are important legume crops yet their rhizobia have not been well characterized. In the present study, 62 rhizobial strains isolated from the root nodules of these plants grown in the subtropical region of China were analyzed via a polyphasic approach. The results showed that 90% of the analyzed strains belonged to or were related to Bradyrhizobium japonicum, Bradyrhizobium liaoningense, Bradyrhizobium yuanmingense and Bradyrhizobium elkanii, while the remaining represented Rhizobium leguminosarum, Rhizobium etli and Sinorhizobium fredii. Diverse nifH and nodC genes were found in these strains and their symbiotic genes were mainly coevolved with the housekeeping genes, indicating that the symbiotic genes were mainly maintained by vertical transfer in the studied rhizobial populations.

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Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China Rhizobia are well known for their ability to carry out symbiotic nitrogen fixation by forming nodules on the roots or occasionally on the stems of legume plants. A total of 1485 leguminous species in 172 genera have been recorded in China, and they are distributed in the tropical, subtropical, temperate and frigid climate zones, from the south to the north. Diverse rhizobia isolated from Chinese legumes have been characterized and several novel species have been proposed (Chen et al., 1988(Chen et al., , 1991Gao et al., 2004;Scholla & Elkan, 1984; Tan et al., 2001;van Berkum et al., 1998;Wang et al., 1999;Wei et al., 2002Wei et al., , 2003Xu et al., 1995;Yao et al., 2002). In addition, novel rhizobial lineages are becoming apparent when further studies are carried out to characterize new isolates with enlarged host ranges and geographical origins.Trees of the genus Albizia, belonging to the Mimosoideae, are native to Asia and Africa and have many uses. In addition to use as foliage, in gardening/landscaping, as green manure and as a source of timber for furniture production, the bark of Albizia trees is used in herbal medicine and the seeds are a source of oil. There are about 150 species in the genus and 17 of them can be found in the southern regions of China (Wu et al., 1988). Although trees of the genus Albizia are of great importance, little is known about the diversity of their microsymbionts. In our previous study, 31 rhizobial strains isolated from Albizia species were characterized by a polyphasic taxonomic approach and greatThe GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains CCBAU 61158 T , CCBAU 61161 and CCBAU 61156 are DQ100066, DQ311088 and DQ311089, and those for the partial glnII, atpD, nodC and nifH gene sequences of strain CCBAU 61158T are respectively DQ311090-DQ311093.A dendrogram based on whole-cell protein patterns, an alignment of the insertion within the 16S rRNA genes of the novel strains and results of DNA-DNA hybridization are available as supplementary material with the online version of this paper.

Thirteen novel strains were isolated from root nodules of three different leguminous plants of the genera Albizia, Kummerowia and Dalbergia grown in China. Cells of these strains were Gramnegative, strictly aerobic, non-spore-forming, motile rods. Phylogenetic analysis of 16S rRNA gene sequences revealed that they belong to the genus Rhizobium.

Traditional evaluation methods do not consider that e-commerce credit evaluation is inherently uncertain and ambiguity .This kind of method makes the evaluation results unnatural. In this paper, a model of e-commerce credit evaluation based on cloud theory is presented. It can transfer quantitative data to qualitative data by cloud transform and cloud maker and matches the index for rules by cloud reasoning engine. If this model is used to explain the result of evaluation, the result could be easier to understand.

The virtual instrument technology and auto test system software architecture for the Signal-Oriented test are first introduced, and then the requirement of virtual instrument driver module is analyzed. Finally, the design scheme of virtual instrument driver module based on Component Object Model component technology is given. Using the scheme, we developed 22 kinds of the test instruments driver. Experiment results show that the scheme improved the driver scalability, maintainability and reusability greatly.

Coiling temperature, finish rolling temperature and running speed of ZSAC1 strip during U-type cooling and number of valves which had been turned on were analyzed in order to find out the reason of low coiling temperature at tail of ZSAC1 strip in U-type cooling process. Results of research showed that running speed of strip and finish rolling temperature were main factors affecting accuracy of coiling temperature. Coiling temperature decreased with the increase of running speed of strip. Coiling temperature fluctuation would occur at the same part of strip when finish rolling temperature increased or decreased. Holding rolling speed and rolling temperature of strip stably can improve accuracy of coiling temperature during downstream U-type cooling.