An outbreak of a new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began in December 2019. Accurate, rapid, convenient, and relatively inexpensive diagnostic methods for SARS-CoV-2 infection are important for public health and optimal clinical care. The current gold standard for diagnosing SARS-CoV-2 infection is reverse transcription-polymerase chain reaction (RT-PCR). However, RTPCR assays are designed for use in well-equipped laboratories with sophisticated laboratory infrastructure and highly trained technicians, and are unsuitable for use in under-equipped laboratories and in the field. In this study, we report the development of an accurate, rapid, and easy-to-implement isothermal and nonenzymatic signal amplification system (a catalytic hairpin assembly (CHA) reaction) coupled with a lateral flow immunoassay (LFIA) strip-based detection method that can detect SARSCoV-2 in oropharyngeal swab samples. Our method avoids RNA isolation, PCR amplification, and elaborate result analysis, which typically takes 6–8 hours. The entire CHA-LFIA detection method, from nasopharyngeal sampling to obtaining test results, takes less than 90 minutes. Such methods are simple and require no expensive equipment, only a simple thermostatically controlled water bath and a fluorescence reader device. We validated our method using synthetic oligonucleotides and clinical
samples from 15 patients with SARS-CoV-2 infection and 15 healthy individuals. Our detection method provides a fast, simple, and sensitive (with a limit of detection (LoD) of 2,000 copies/mL) alternative to the SARS-CoV-2 RT-PCR assay, with 100% positive and negative predictive agreements.
To analyze the influencing factors and outcomes of the different severity of diabetic ketoacidosis (DKA).
A total of 50 children with DKA admitted to the Department of Pediatrics, Tianjin Medical University General Hospital from January 2009 to December 2018 were included in this study. The patients were divided into mild group, moderate group, and severe group according to the severity of the disease. We then analyzed the clinical characteristics and outcomes of the 3 groups.
Compared to mild and moderate DKA groups, patients with severe DKA were more likely to present chest tightness, and higher levels of blood osmotic pressure, urea, and creatinine (
P
< .05). Logistic regression analysis showed that blood osmotic pressure, creatinine, and chest tightness were independent factors for severity of DKA. There was a significant difference in the resolution time of DKA among the 3 groups (mild vs moderate: 9.0 hours vs 15.25 hours; moderate vs severe: 15.25 hours vs 24.5 hours,
P
< .001). There were statistical differences in the decline of Glasgow score among 3 groups (
P
= .004).
Patients with severe DKA showed higher osmotic pressure and creatinine, as well as dyspnea. The children with severe DKA were more likely to present progression of neurological symptoms, which was necessary to pay attention to the presence of brain edema.
Influenza viruses
and respiratory syncytial virus (RSV) have contributed
to severe respiratory infections, causing huge economic and healthcare
burdens. To achieve rapid and precise detection of influenza viruses
and RSV, we proposed a catalytic hairpin assembly (CHA) combined with
the lateral flow immunoassay (CHA–LFIA) detection method. The
presence of the target RNA triggers the initiation of CHA circuits.
H1/H2 complexes, the amplified signal products, which were labeled
with digoxin and biotin, were detected with a highly sensitive lateral
flow immunoassay system. The sensitivity of the CHA–LFIA system
to influenza A and B viruses and RSV reached up to 1, 1, and 5 pM,
respectively. In addition, this method exhibited excellent capability
for differentiating between target RNA and base-mismatched RNA. The
results demonstrated that an enzyme-free, rapid, highly sensitive,
and specific method had been developed to detect influenza A and B
viruses and RSV.
With the deepening of the double reduction policy, the current school management pays more and more attention to adopting flexible teaching management mode and permeating the people-oriented management concept. Based on this, school administrators should handle all kinds of interpersonal relationships in schools under the guidance of people-oriented and strive to create a harmonious, relaxed and free interpersonal atmosphere, and strive to build good school interpersonal relationships, so as to arouse the enthusiasm of teachers' work to the greatest extent.Infiltrating people-oriented into the process of organizing and implementing management forms can help organize management functions, and further strengthen the management work can also make it effective. In the process of campus management, we should recognize the core of personnel management, focus on building a people-oriented management framework, optimize interpersonal relationships, and build a good campus interpersonal atmosphere.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.