The fragmentation mechanisms of three types of brassinosteroids (BRs), 23,24-tris-epicastasterone, epicastasterone, tris-epicastasterone, 24-epibrassinolide and 6-deoxo-24-epicastasterone, have been extensively investigated by tandem mass spectrometry (MS(n), n = 1, 2, 3, 4, 5) with the assistance of high mass accuracy quadrupole time-of-flight mass spectrometry (QToF MS). The electrospray ionization (ESI) mass spectrometric fragmentation pathways of these five BRs were comprehensively elucidated for the first time. Cleavages of side chains, neutral losses of water or other molecules and opening of a ring induce the main fragmentation patterns. The results from the present study can potentially afford important guidance for the structural elucidation of different BRs and provide some fundamental data for metabolomic analysis of BRs.
The fragmentation pathways of two isomers of brassinosteroids, 28-homobrassinolide (28-h-BL) and 28-epihomobrassinolide (28-eh-BL), have been investigated by tandem mass spectrometry (MS n , n = 1, 2, 3, 4) with the electrospray ionization (ESI) source. The ESI mass spectrometric fragmentation pathways of protonated 28-h-BL and 28-eh-BL were comprehensively elucidated, and the principles revealed in this investigation could potentially be used to identify and distinguish brassinosteroids and their isomers.
brassinosteroids, brassinolides, fragmentation, ion-trap tandem mass spectrometry Citation:Huo F F, Bai Y, Liu H W. Fragmentation study of two brassinolides by ion trap tandem mass spectrometry.
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