Abstract. Andayani S, Dadiono MS, Elwira WT, Setyawan FH. 2020. Potency of aloe extract as immunostimulant for carp (Cyprinus carpio) against Aeromonas salmonicida. Biodiversitas 21: 860-864. The objective of the study was to be able to know the cellular and humoral non-specific immune response to Aeromonas salmonicida after aloe extract administration. This study employed Complete Randomized Design with 3 treatments and 3 replications, control treatment (K), A =2 ppm of aloe extract application in the media, B = 16 ppm of aloe extract in the media, and C = 30 ppm of aloe extract in the media. The extract was given through one-hour soaking at day 0 and day 7, and then challenge-tested to 107 cells.ml-1 of A. salmonicida into the media for one day. Results showed that administration of aloe extract could raise plasma protein levels from 443.0 to 662.0 mg.Kg-1 antibody from 1.2576 to 3.3974, and phagocytic activity from 30.61% to 45.94%. Similarly, the differential value of leucocyte (lymphocyte from 58.6% to 73.70%, neutrophils from 6.09 to 8%, and monocyte from 5.70% to 7.72%). As conclusion, aloe extract at the dose of 2 ppm could raise the cellular and humoral non-specific immune. Therefore, the use of aloe extract as immunostimulant at the dose of 2 ppm applied through immersion could also apply other methods, such as oral and injection.
Dampak dari penggunaan antibiotik dapat menimbulkan masalah pada kegiatan budidaya seperti resistensi bakteri dan bersifat residu bagi tubuh konsumen. Oleh karena itu, salah satu alternatif yang dapat digunakan untuk menghambat atau membunuh bakteri dengan menggunakan plankton Chaetoceros calcitrans. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak C. calcitrans yang dapat menghambat atau membunuh bakteri dan mengetah ui perlakuan dengan dosis terbaik dalam menghambat pertumbuhan bakteri A. salmonicida. Metode yang digunakan adalah metode eksperimental dengan Rancangan Acak Lengkap (RAL) yang terdiri dari 4 perlakuan, 3 ulangan dan 2 kontrol. Perlakuan A (0.3 ppm), perlakuan B (15.3 ppm), perlakuan C (30.3 ppm), perlakuan D (45.3 ppm), kontrol positif (chlorampenicol 5 ppm) dan kontrol negatif (tanpa diberi ekstrak). Hasil penelitian menunjukkan rata-rata diameter zona bening tertinggi terdapat pada perlakuan D dengan nilai 5.37 mm dan hasil zona bening terendah terdapat pada perlakuan A dengan nilai 2.89 mm. Penambahan dosis perlakuan ekstrak C. calcitrans terhadap diamater zona hambat menunjukkan pola linier dengan persamaan y = 3.2067 + 0.0509x dan koefisien R 2 = 0.9262. Hubungan antara pemberian ekstrak C. calcitrans dalam menghambat pertumbuhan bakteri A.salmonicida dengan rata-rata diamater zona hambat yang dihasilkan menunjukkan respon yang meningkat seiring dengan bertambahnya dosis ekstrak. Dari penelitian menunjukkan bahwa ekstrak C. calcitrans dapat menghambat pertumbuhan bakteri dan dosis dengan hasil tertinggi terdapat pada perlakuan D dengan dosis 45.3 ppm.
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