Helicobacter pylori (H. pylori) are Gram-negative bacteria that colonize human gastric mucosa, leading to chronic gastritis. Parasitic infections are associated with H. pylori infection. This work aims is to evaluate the association of H. pylori and/or intestinal parasites infection with anemia in Egypt. Blood and stool specimens of 32 Subjects: 17 males and 15 females, whose age ranged from 1.5-65 years were analyzed for H. pylori antigen using Rapid Anti H. pylori test. Stool specimens were also examined microscopically for the presence of parasite eggs or larvae/cysts per wet amount by the saline concentration method. Complete blood counts (CBC) were calculated using Blood Cell Counter. The results indicated that out of the collected 32 samples 17 (53.1%) were males and 15 (46.9%) were females; 22 (68.8%) were infected (11 with H. pylori only (G1) and 11 with H. pylori and/or intestinal parasites (G2), 34.4% each) and 10 (31.2%) were not infected (control group). In the parasitic infected G2: 7 (63.6%) were infected with protozoa and 3 (27.3%) were infected with helminthes and 1 (9.1%) has mixed infection with both protozoa and helminthes. Highly significant changes (p< 0.05) were observed for Hemoglobin level, RBCs count, Hct, Mean Cell Volume (MCV) and Mean Cell Hemoglobin (MCH) that were measured in G1 and G2 than in the control. In conclusion, there are high prevalence of H. pylori and intestinal parasites infections and this was highly associated with anemia disease in the studied subjects.
Generally, the parasitological markers showed that Neem (Azadirachta indica) and Mirazid (Commiphora molmol, MZ) were effective in reducing worms and eggs of Schistosoma mansoni. This study aimed to detect the histopathological improvement accompanied with using Neem and Mirazid to treat schistosomiasis. A total of 120 Swiss Albino mice were categorized into 10 groups: 5 groups infected with S. mansoni and either treated with only Neem (400 mg/kg), only MZ (500mg/kg), a combination of Neem and MZ, praziquantel (PZQ, 200 mg/kg) or not treated. Other 5 control groups were not infected but acquired the same treated doses as the above groups. The study was done in
Two field experiments were carried out at Mallawy Water Requirements Research station -El Minia, Governorate; Egypt Water Management Research Institute -National Water Research Center during 2012 and 2013seasons. The present research was carried out to study the effect of water stress and planting methods on yield, saving water and quality for Potato crop (Solanum tuberosum L). A split plot design with three replicates was conducted. The main plots were assigned to five irrigation treatments traditional irrigation (the farmers practices), 100%, 90%, 80% and 70% of field capacity for potato crop and the sub plots into two planting methods (furrow and beds). The treatments of irrigation were distributed at random in the main plots. While planting methods treatments were distributed at random in the sub-plots. Results indicat that irrigation regime significantly affected total yield in both seasons, where values of total yield/fed. were increased as water stress increased until 80% of field capacity then decreased with 70% of field capacity in both seasons. Also planting method was significantly affected this character in both seasons. The highest yield of potato was obtained with planting in beds and saving water about 14.42% compare to planting in furrow in both seasons. The obtained results in present study show that when the best method was used (irrigated potato until 80% of field capacity and planting in beds A 4 b 2 ) the irrigation water is saved more than the normal planting in furrow (common method in region) by about 29.72% in both seasons. This treatment was the most superior treatments (from view point water and production) on this character. Starch content values were significantly increased as water stress was decreased where the highest values of starch content (based on dry weight) were obtained when plants irrigated by conventional irrigation (72.820 and 74.400) in the first and second seasons, respectively. Planting method significantly affected this character in both seasons, where the highest values were obtained from plants which planting in beds in both seasons. The interactions between the studied factors significantly affected strach content in both seasons. Irrigation regime significantly affected the protein content in both seasons, where the highest value was obtained from plants which irrigated with conventional irrigation (6.958 and 7.035%) in the first and second seasoins, repectively. Irrigation regime significantly affected total soluble solids percentage (TSS) and dry matter content in both seasons , where they were increased with increasing water stress in both seasons, and the maximum values of (TSS) and dry matter content were recorded when plants grown at the lowest level of water supply i.e. irrigation at 70% depletion of available water. Irrigation regime was significantly affected the specific gravity (g/cm 3 ) of potato in both seasons, where the specific gravity was increased significantly as watrer stress was increased. Planting methods significantly affected on this chara...
The present study investigated the possible protective effects of melatonin on Bleomycin, Cisplatin and etoposide (BEP) chemotherapy regimens using immunohistochemistry. Forty male Wistar rats were divided into four groups of ten as; group 1 as untreated control; group 2 as BEP group which received the three cycles of 21 days' regimen each of 0.5¥ dose levels ofBEP (bleomycin 0.75 mg/kg, etoposide 7.5 mg/kg and cisplatin 1.5 mg/kg). Rats in the group 3 (MEL group) received 10 mg/kg/day melatonin once daily. Group 4 received the melatonin (30 min before the BEP injections) and BEP as in groups 2. Proliferating cell nuclear antigen (PCNA) staining was used to detect cell proliferation and caspase-3, caspase-9 and Caspase-8 were detected to investigate apoptosis. PCNA immunostaining in alveolar epithelium, alveolar macrophages and bronchus was weak to moderate in BEP group. However, diffuse and strong caspase immunoreactions for caspase-3, caspase 8-and caspase-9 were detected in the bronchioles epithelium, vascular endothelium, alveolar luminal macrophages in the BEP group. PCNA and caspase immunoreactivities in MEL and Mel + BEP groups were close to the control one. The surface are in the BEP group was significantly reduced as compared to the control one ((P<0.05). However, in MEL and Mel + BEP groups, it was close to the control value (P>0.05). It can be concluded that BEP regimen can affects negatively on lung tissue and melatonin inhibits lung tissue injuries during BEP chemotherapy.
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