Electrolytes are of great importance in cell metabolism because they are important cofactors or coenzymes needed for normal functioning of cells. The disturbances of these electrolytes result in changes of cell pH and acid base balance. In order to determine the electrolytes changes following feed and water deprivation in male rats, a total of ten (n=10) male albino rats weighing (152 ± 3.50)g housed in metallic cage were used for this study. The rats were deprived of feed and water consecutively for six days. On the third and sixth days blood samples were obtained for electrolytes studies in the plasma and in the erythrocytes. There were significant increases (P<0.05) in plasma sodium, potassium, calcium, magnesium, chloride and bicarbonate concentrations in comparison to the basal parameters while significant decreases (P<0.05) in erythrocytes sodium, potassium, calcium, magnesium, chloride and bicarbonate concentrations in comparison to the basal parameters were observed. It can be concluded that deprivation of male albino rats of food and water for six consecutive days resulted in perturbation, stress, dehydration and redistribution of electrolytes in various compartments of the cell.
SummaryThis study investigated the breeding parameters and embryogenic development of diploid and heat shock-induced triploid eggs of Anabas testudineus (Bloch, 1792). To this effect, broodstocks of A. testudineus were induced to spawn using the Ovaprim® hormone. After fertilization, the eggs were divided into two groups and one portion heat shocked at 41°C (for 3 min), at approximately 4 min after fertilization. Results of fertilization, hatchability, as well as the sequence and timing of embryogenic development were collated from three breeding trials. Fertilization percentages were similar in both treatments (≈90%) while hatchability was higher in the diploid eggs (79.56%) than the triploid induced eggs (50.04%). Both treatments had the same sequence of embryogenetic stages; however, the timing of development was significantly delayed in the triploids (i.e. beyond the 2-cell stages) as compared with the observations in the control group (diploid eggs). Consequently, hatching time was 5 h faster in the diploid eggs [i.e. 18 hours post fertilization (hpf)] compared with the triploid induced eggs (23 hpf). The most critical stage of embryonic development in which mass mortality occurred in the different treatments was the somite stage. The status of triploid hatchlings was affirmed using erythrocyte morphology in 2-month-old fingerlings.
This study was carried out to investigate the changes in semen characteristics, hormonal profile and testicular morphometry of West African Dwarf (WAD) goat bucks treated with danazol. For this purpose, eighteen matured WAD bucks average of about two years were randomly divided into groups A, B and C comprising of six bucks per group. Group A was the control while B and C bucks were given danazol at a dose rate of 20mg/kg body weight orally daily for four weeks. The group B bucks’ testes were harvested thereafter for gross and histo-morphometric studies while those of group C were left intact but danazol treatment was withdrawn for four more weeks. The bucks’ semen samples (collected by electro-ejaculation) and hormonal samples (taken via the jugular vein) were analyzed. The semen characteristics studied included color, volume, mass activity, motility, percentage normal live-dead ratio, morphology and concentration; while the hormones studied included testosterone, follicle stimulating hormone and luteinizing hormone. Results show that there were significant decreases in semen characteristics of group B and C bucks compared with those of group A in the first four weeks. The semen characteristics of the group C bucks were reversed to normal range (similar to those of group A bucks) two weeks after danazol treatment was withdrawn. Similar results were observed with the hormonal studies. In conclusion, danazol caused a reversed reduction in sperm cells characteristics suggesting its possible use as a contraceptive in WAD goat bucks.
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