Medicinal plants contain a wide variety of chemicals which have very important roles in numerous applications including medicinal and those related with industry. Essential oils represent valuable sources for natural antioxidants. The aim of our study was to evaluate the chemical composition and antioxidant activity of the essential oils extracted from leaves of Syrian Vitex agnus-castus L. and Rosmarinus officinalis L., where is both essential oils were extracted and analyzed by gas chromatography-mass spectrometry. The antioxidant activities of these essential oils were determined by three different test systems, scavenging effect on 2,2-diphenyl-1-picrylhydrazyl radical, total phenolic and flavonoids contents. The main constituents found in Vitex agnus-castus L. essential oil were 1,8-Cineole (19.34%) and Sabinene (12.50%), while the major constituents in Rosmarinus officinalis L. essential oil were 1,8-Cineole (28.03%) and α-Pinene (14.70%). The results showed that 2,2-diphenyl-1-picrylhydrazyl radical scavenging and total phenolic contents of Vitex agnus-castus L. essential oil were higher than Rosmarinus officinalis L. essential oil. Total flavonoids contents were not detected in both essential oils. The Vitex agnus-castus L. and Rosmarinus officinalis L. essential oils are sources of natural antioxidants. Therefore, further work is needed to identify the compound(s) responsible for the antioxidant activity of Vitex agnus-castus L. and Rosmarinus officinalis L. essential oils.