Objectives: Toothpastes and mouthwashes contain ingredients that may be toxic for oral mucosal tissues. This study aimed to assess the cytotoxicity of the ingredients of commonly used toothpastes and mouthwashes. Materials and Methods: This experimental study was performed on 16 toothpastes and four mouthwashes widely available in the Iranian market. First, the concentration of six main ingredients of these products, namely sodium fluoride (NaF), sodium lauryl sulfate, cocamidopropyl betaine, zinc lactate, paraben, and sodium benzoate, was determined. The methyl thiazolyl tetrazolium (MTT) assay was used to assess the cytotoxicity of these materials for human gingival fibroblasts (HGFs). The MTT assay was performed at 1, 15, and 30 minutes following exposure to five concentrations of each material in triplicate (according to the concentrations obtained in the isolation step). Data were analyzed using three-way analysis of variance (ANOVA). Results: The difference in the cytotoxicity of the materials was statistically significant (P<0.001). Cytotoxicity was time- and concentration-dependent; by an increase in the concentration of the materials, their cytotoxicity increased over time. The cytotoxicity of sodium lauryl sulfate and cocamidopropyl betaine was >90%. The cytotoxicity of NaF varied from 25% to 70%, and the cytotoxicity of all concentrations of zinc lactate and sodium benzoate was <50% for HGFs. Conclusion: To decrease the cytotoxic effects of toothpastes, sodium lauryl sulfate and cocamidopropyl betaine should be replaced with safer detergents, and the concentration of fluoride should be decreased to 400 parts per million (ppm). Alternatively, fluoride may be replaced with other antibacterial and cariostatic agents.
Background. Aesthetic restorations should be able to mimic the natural colour depth of teeth, affected by several factors including material properties. There is a lack of information regarding the effect of cement shade and material thickness on the final colour of ultratranslucent multilayered zirconia veneers. Objectives. This study evaluated the effect of ceramic thickness and resin cement shade on the final colour of different layers of ultratranslucent multilayered (UTML) zirconia veneers. Methods. This in vitro study produced 90 rectangular-shaped specimens with nonsintered Katana UTML monolithic zirconia (Kuraray Noritake Dental, Tokyo, Japan), shade A1 blocks. Ceramic samples were prepared in two groups of 0.7 mm and 0.5 mm thicknesses, 45 of each (a: 8 × 11 × 0.5 mm ; b: 8 × 11 × 0.7 mm ). Specimens of each thickness were further divided into 5 groups: universal, clear, brown, white, and opaque ( n = 9 ). Each adhesive resin cement (Panavia V5) was applied between the ceramic samples and composite substrate. The colour values were measured using a spectrophotometer in baseline and after resin cement application according to the CIELab system. For all samples, Δ E 00 values were obtained. Data were evaluated with SPSS 25 using the three-way ANOVA test ( p < 0.05 ). Results. The factors of cement shade, ceramic thickness, and ceramic layers have statistically significant effect on Δ E 00 values ( p < 0.001 ). The results showed lower Δ E 00 values with thicker ceramic veneers. Tukey test results showed that the opaque and brown shade had a significantly greater Δ E 00 values comparing to universal ( p = 0.004 ), clear, and white shades ( p < 0.001 ). Conclusion. The colour change was greater in lower ceramic thickness. Different shades of resin cement and layers of UTML zirconia differently affected the final colour.
Purpose. Debonding from the root canal dentin is the most common failure mode of fiber posts. This study aimed to assess the effects of cold atmospheric argon plasma (CAAP) and ethylenediaminetetraacetic acid (EDTA) on micro-push-out bond strength of fiber posts to root canal dentin. Materials and Methods. Forty maxillary canine teeth were decoronated, underwent endodontic treatment, and were stored in an incubator for 7 days. After post space preparation, the teeth were randomly divided into four groups for different surface treatments: (I) saline, (II) 17% EDTA, (III) CAAP, and (IV) 17% EDTA + CAAP. Fiber posts (Whitepost no. 2, FGM) were cemented into the root canals using Panavia F2.0 resin cement, and 1 mm-thick sections were made at the coronal, middle, and apical thirds of the roots. The samples underwent micro-push-out bond strength test. The mode of failure was also determined under a stereomicroscope. Data were analyzed using three-way ANOVA and Tukey’s post hoc test (α = 0.05). The mode of failure data were analyzed using the chi-square test. Results. The mean micro-push-out bond strength of fiber posts was not significantly different in the four groups ( P > 0.05 ). However, the bond strength values in the coronal third were significantly higher than the corresponding values in the apical third ( P = 0.01 ). There was no significant difference in the modes of failure between the groups ( P > 0.05 ). Conclusion. Application of CAAP alone or in combination with 17% EDTA could not successfully increase the bond strength of fiber posts to root canal dentin.
Background: Limited curing depth and its effect on the degree of conversion are among the challenges of working with light-cure composite resins. The use of bulk-fill composites is one strategy to overcome these limitations. Methods: Ever X Posterior (EXP), Filtek Bulk-Fill Posterior (FBP), Sonic Fill 2 (SF2), Tetric N-Ceram Bulk-Fill (TNB), and X-tra Fil (XF) bulk-fill and Filtek Z250 conventional composite were evaluated in this in vitro experimental study. Six samples for the assessment of microhardness and three samples for the evaluation of DC were fabricated of each composite. After light curing and polishing, the samples were incubated at 37°C for 24 hours. Microhardness was measured by a Vickers hardness tester three times and the mean value was calculated. DC of the top and bottom surfaces was determined using Fourier-Transform Infrared Spectroscopy (FTIR). Data were analyzed using one-way ANOVA and Tukey’s test. Results: Microhardness and DC were significantly different among the groups (P<0.001). XF and Z250 equally showed the highest bottom-to-top surface microhardness ratio (0.97 ± 0.01) and significantly higher DC in the top (P<0.001) and bottom (P<0.005) surfaces compared to other groups. TNB showed the lowest microhardness ratio (0.88 ± 0.04) and DC (68.66 ± 1.52 and 61.00 ± 2.00); the difference in DC of the bottom surface was statistically significant (P<0.003). Conclusion: It appears that bulk-fill composites evaluated in this study are adequately polymerized at 4 mm depth. Their DC was optimal and within the range of conventional composites.
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