In this study the effects of zinc sulfate and gibberellin on agro physiological of white bean under water deficiency were studied. Therefore, an experiment was conducted in a split-split plot design based on a randomized complete block with three replications in two places. The experimental factors included three irrigation levels, spraying of zinc sulfate in four levels and two levels of non-spraying and spraying of gibberellin. Analysis of measured data indicated that the water stress had a significant effect on all traits, except proline amount and 100 seeds weight. Spraying of zinc sulfate showed a significant effect on all traits except carotenoid value. Application of gibberellin had a significant effect on all traits except ion leakage, carotenoids, number of seeds per pod and grain yield.The interaction effect of stress×zincsulfate×gibberellin was significant on all traits except number of seeds per pod. In addition, comparison of means at 5% level, showed that application of 1.5 ml L -1 of zinc sulfate plus gibberellin improved bean biochemical properties. Under optimum water level, using of 4.5 ml L -1 of zinc sulfate and under severe water stress conditions, using of 4.5 ml L -1 of zinc sulfate plus gibberellin are recommended for obtaining the maximum crop performance.
ABSTRACT:In this study, five biochemical traits obtained for seedlings of five different provenances of Quercus castaneifolia C.A. Meyer were used to evaluate the available light environment using instantaneous electronic readings as a standard. Experimental design was executed under controlled conditions at six different irradiance levels (10, 20, 30, 50, 60, and 70% of full light). Results show that among total chlorophyll (Chl mass ), chlorophyll a to b ratio (Chl a :Chl b ), N, chlorophyll to N ratio (Chl:N), and peroxidase (POD) quantitative activity, the latter provides the highest realistic correlation with available irradiance. Ranking irradiance treatments on a Chl mass basis, Chl:N ratio, and Chl a :Chl b ratio do not consistently distinguish different irradiance levels either within or between provenances, while the ranking produced by POD was an accurate reflection of the degree of irradiance within the shade houses, especially at 10-30%.
ABSTRACT:To understand the function of peroxidase (POD) in relation to a light gradient, changes in POD activity were studied in five different provenances of chestnut oak seedlings. An experiment was conducted in controlled conditions and six different irradiances (10, 20, 30, 50, 60, and 70% of full light) were considered. According to the results, POD activity was strongly related to irradiance and showed a decreasing response to light, with the largest changes at low light (10 to 30%) and a levelling-off at high light (50-70%). Five provenances were also significantly distinguished at 10-30% of full light. The gel analysis showed that there were several different bands between irradiances from 10 to 70% regardless of provenances. One isoenzyme with the highest electrophoretic mobility was significantly increased in response to reduced light and slightly decreased at high irradiance. Other isoenzymes were significantly increased at high irradiance, suggesting that these isoenzymes are most likely involved in response to light stress and leaf adaptation to high irradiance.
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