Abstract:Bacterial infection is an important complication among children with cancer, who are under chemotherapy. Bacterimea may occur during chemotherapy, which is an invasive procedure and also due to reduced number of neutrophil count. The study was conducted in a tertiary level hospital, Bangabandhu Sheikh Mujib Medical University in Bangladesh from March to September 2010. The aim of this study was isolation and identification of bacteria from blood culture among children with febrile neutropenia who were under chemotherapy. Blood samples were collected from 30 febrile neutropenic children and culture was done by traditional culture method using Trypticase soy broth for primary culture. Out of 30 blood samples, culture were positive in 6(20%) and were negative in 24(80%) samples. Two third of the culture positive patients had absolute neutrophil count (ANC) less than 100 cells/cmm. The isolated organisms were Pseudomonas spp (33.3%) Staphylococcus aureus (33.3%), Esch. coli (16.7%) and Acinetobacter spp (16.7%). Amikacin, imipenem and meropenem were the most effective antibiotics having 100% sensitivity. Blood culture is essential to evaluate the febrile neutropenic children who were under chemotherapy.Key word: Antibiotics, Bangladesh, Blood culture, Febrile neutropenia.
Pseudomonads are the most important gram negative organisms involved in various types of infection. This cross sectional study was conducted from January to December 2010 to isolate and identify Pseudomonad at species level in different clinical samples by culture and multiplex polymerase chain reaction (PCR) and to evaluate the efficacy of PCR in rapid detection of the bacteria at species level. Wound swab and tips of endotracheal tube were collected from hospitalized patients from different surgical units and intensive care unit (ICU) of Dhaka Medical College Hospital, Dhaka. Pseudomonads were isolated and identified at species level by culture, microscopy, different biochemical tests and PCR. Among 230 samples, 52.6% were surgical wound, 34.3% were burn wound and 9.6% were traumatic wound samples and 3.4% were tips of endotracheal tubes. Twenty six percent isolated organisms were Pseudomonas spp., 30.4% were Escherichia coli, and 13.5% were Staphylococcus aureus. Others were Proteus, Klebsiella pneumoniae, Acinetobacter baumannii and Enterobacter spp. In 19.67% samples mixed infections by other organism (Esch coli, Staph aureus, Proteus spp, Klebsiella spp) with Pseudomonas were detected and its distribution was highest in traumatic and burn wound. Multiplex PCR and different biochemical tests were used to identify 3 bacterial species of Pseudomonad. Among the species identified, 95.52% was Pseudomonas aeruginosa, 2.99% was Stenotrophomonas maltophilia and 1.49% was Burkholderia cepacia. The sensitivity of multiplex PCR was 95.08% and specificity 94.67%. PCR was the most rapid and more accurate method for detection of Pseudomonad at species level.
Bangladesh J Med Microbiol 2016; 10 (2): 22-26
Fever is the commonest symptom of infection in neutropenic children with malignancy, under chemotherapy. To avoid bacterial complications, it is necessary to diagnose infection early and to administer empirical antibiotic. The aim of this study was to find out the causative bacteria and their antimicrobial susceptibility pattern in febrile neutropenic children who were under chemotherapy. The study was conductedin a tertiarylevel hospital,Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. Blood, urine and wound swab were collected for culture from 30 febrile neutropenic children, age 1-15 years. Out of 70 samples total 26(37.14%)yielded growth of bacteria among which 6(20%), 12(40%), and 8 (80%) were isolated from blood, urine and wound swab respectively. Among thebacteria Pseudomonas spp, Esche coli and Staph aureus weremost frequently isolated. Meropenem, imipenem and netilmicin were the most sensitive against gram negative bacteria and the entire isolated gram positive bacteria were 100% sensitive to vancomycin and linezolid.
Bangladesh J Med Microbiol 2017; 11 (2): 5-8
The clinical condition of the patient, type of central venous catheter (CVC), site and duration of CVC placement are the factors affecting the risk of infection. The aim of this study was to examine and find out the risk factors of CVC related blood stream infections (CVC-BSI). This cross sectional study was carried out in the Department of Microbiology and Immunology of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh during the period of July 2011 to June 2012. One hundred patients who were admitted in ICU of BSMMU and ICU and haemodialysis unit of Dhaka Medical College Hospital (DMCH) having central venous catheter, were enrolled in the study. The rate of CVC-BSI was 11% and the incidence was observed to be 11.14/1000 catheter days. Both CVC-BSI and CVC colonization were higher in trilumen than in bilumen central venous catheter. CVC-BSI rate was 12.79% in trilumen whereas there was no CVC-BSI in patient with bilumen catheter. The mean duration from CVC insertion to development of CVC-BSI was 14 days, CVC colonization was 8.41 days and noninfected CVC was 6 days. CVC-BSI and CVC colonization were most common in right femoral vein where CVC-BSI was 18.52% and CVC colonization was 59.26%, whereas no CVC-BSI was found in right internal jugular vein. Risk factors for CVC-BSI included type of CVC, site of CVC placement, duration of catheterization were not found statistically significant in this study. CVC-BSI and CVC colonization were higher in trilumen catheter and rate raised with increased duration of placement and highest number of CVC-BSI and colonization was found in right femoral vein.
Bangladesh Med J. 2018 Jan; 47 (1): 18-21
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