The present investigations were carried out to establish the medicinal value of Argyrolobium roseum synonym Cytisus roseum. It is a medicinal plant, belongs to family Leguminoseae. The antioxidant potentials of the different fractions of A. roseum were screened by using ABTS •+ assay, DPPH free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity by phosphomolybdenum method, total phenolic contents determination, total flavonoid contents and metal chelating activity of ethyl acetate, n-hexane, chloroform, methanol and aqueous fractions. ABTS •+ assay results reveal that ethyl acetate soluble fraction demonstrated the good antioxidant activity having the highest Trolox equivalent antioxidant capacity (TEAC) value i.e., 152.43 ± 0.43 mM/µL Trolox equivalent and the highest total flavonoid contents having total flavonoid content value 931.91 ± 0.06 mg/g QE, n-hexane soluble fraction showed the highest percentage of metal chelating activity 61.85 ± 0.9 % bound iron and highest total phenolic contents 792.5 ± 0.55 gallic acid equivalent mg/g whereas aqueous extract indicated the ferric reducing antioxidant power value 70.48 g/mL. It was concluded from antioxidant screening that ethyl acetate soluble fraction was rich in strong radical scavenging active components but chloroform soluble fraction and aqueous extract were valuable resources of phenolics and flavonoids.
The present investigation deals with the determination of various serum enzymes known to be elevated during myocardial infarction (MI) and estimation of selected metals like Cu, Cr, Co, Fe, Pb, and Mg by flame atomic absorption spectrophotometry. The data obtained thereby were processed for the determination of correlation coefficient matrix among the cardiac enzymes and the serum metals. The study evidenced the accumulation of Pb during MI and reduction in the level of Fe. A significant negative correlation was observed between Cu and creatine kinase-MB. The data were also segregated into various groups to study the influence of age and gender on the levels of selected parameters. In both the genders, the age of the patients was found to be correlated significantly with various cardiac enzymes. In case of male patients, the most significant correlation was observed between age and blood sugar at random. The other significant correlations among the male patients included Cr-CPK, Cr-creatine kinase-MB, Fe-age, and others. In female patients, the pairs of studied parameters that exhibited significant correlations included age-lactic dehydrogenase, creatine phosphokinase isoenzyme-aspartate aminotransferase, lactic dehydrogenase-creatine phosphokinase isoenzymes, Pb-Fe, and Cu-Co in addition to others.
Objective
The aim of the present investigation was to assess the antioxidant, antibacterial and antifungal activities of various fractions of Wendlandia exserta (Roxbukan Pansara).
Methods
The methanolic extract of W. exserta (Roxbukan Pansara) was dissolved in water (distilled) and then partitioned with solvents e.g. n-hexane, CHCl3, EtOAc and n-BuOH consecutively. Antioxidant potential of above mentioned fractions and remaining aqueous fraction was checked by four methods. Antimicrobial activity was examined by four bacteria and four fungi by the disc diffusion method by using a standard, as positive control.
Results
The aqueous fraction displayed maximum value of diphenyl picryl hydrazyl (DPPH) radical inhibition (85.94%±0.35) with an IC50 value of 415.31±0.26 μg mL−1. The aqueous fraction also displayed the maximum ferric reducing antioxidant power (FRAP) value [64.5±0.15 μg of trolox equivalents (TE)] as well as maximum inhibition of lipid peroxidation (42.32%±0.59) and maximum value of total phenolic contents (87.10±1.10). However, the n-BuOH fraction was found to display the maximum value of total antioxidant activity (0.529±0.76). The fractions also displayed good antimicrobial activity against various microorganisms.
Conclusions
It was concluded that polar fractions of W. exserta showed good antioxidant and antimicrobial activity, so these might be useful in pharmacological preparations.
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