The fingerprinting DNA method which depends on the unique pattern in this study was employed to detect the hydatid cyst of Echinococcus granulosus and to determine the genetic variation among their strains in different intermediate hosts (cows and sheep). The unique pattern represents the number of amplified bands and their molecular weights with specialized sequences to one sample which different from the other samples. Five hydatitd cysts samples from cows and sheep were collected, genetic analysis for isolated DNA was done using PCR technique and Random Amplified Polymorphic DNA reaction(RAPD) depending on (4) random primers, and the results showed:
1-Ability of OPF-13 primer to find fingerprinting for DNA of hydatid cysts collected from cows with
(3) unique patterns and from sheep with (5) unique patterns.
2- Ability of OPF-06 primer to found (4) unique patterns for cows and (2) unique patterns for sheep.
3-Ability of OPF-19 primer to find fingerprinting for DNA of hydatid cysts collected from cows (5) unique patterns and (4) unique patterns for sheep.
4-OPF-16 was unable to find the finger prints of DNA isolated from cows and sheep
Keywords : Hydatid cyst. OPF Primer .PCR. RAPD.
Multiple myeloma patients were carried out the period (October/2017-February/2018), for measurement the concentrations of IgM,IgM amd IgG antibodies,types of light chains(kappa and Lambda) alpha-1,2 and gamma as well as
albumin and protein levels in the sera of 47 (25 males and 22 females) patients compared with 29 healthy humans as control group. The diagnosis of patients were done by immunofixation technique.The positive titer of immunoglobulins showed 2(2.6%) IgM
,6(7.6%) IgA and 33(43.4%) IgG, while 6(7.8%) was negative titer. The light chains Kappa 22(28.9%),Lambda 20(26.3%), while 5(6.6%) negative, it was noticeable changes among patients and healthy group. The alpha-1 in patients 19(25%), alpha-2
14(18.5%), beta 9 (11.8%), gamma 37(48.7%) were abnormal in patients of multiple myeloma (observed changes). Abnormal concentration of albumin and protein in the multiple myeloma patients showed 28(36.8%),18(23.6%) respectively also highly observed
changes within patients and healthy group.
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