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Herein we report the development of solid-phase microextraction (SPME) devices designed to perform fast extraction/enrichment of target analytes present in small volumes of complex matrices (i.e. V≤10 μL). Micro-sampling was performed with the use of etched metal tips coated with a thin layer of biocompatible nano-structured polypyrrole (PPy), or by using coated blade spray (CBS) devices. These devices can be coupled either to liquid chromatography (LC), or directly to mass spectrometry (MS) via dedicated interfaces. The reported results demonstrated that the whole analytical procedure can be carried out within a few minutes with high sensitivity and quantitation precision, and can be used to sample from various biological matrices such as blood, urine, or Allium cepa L single-cells.

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Abstract:The current study reports the development of solid phase microextraction (SPME) devices designed to perform fast extraction/enrichment of target analytes present in small volumes of complex matrices (i.e. V ≤ 10 μL). Micro-sampling was performed with the use of etched metal tips coated with a thin layer of biocompatible nano-structured polypyrrole (PPy (tip diameter ≤ 10 µm), or by using coated blade spray (CBS) devices with an extraction phase thickness ≤ 10 µm, facilitating rapid extraction. These devices can be coupled either to liquid chromatography (LC), or directly to mass spectrometry (MS) via dedicated interfaces. The reported results demonstrated that the whole analytical procedure can be carried out within a few minutes (tanalysis ≤ 7 min) with high sensitivity and quantitation precision, and can be used to sample from various biological matrices such as blood, plasma, and urine, towards the analysis of therapeutics, pesticides, and controlled substances as model analytes. As a proof-of-concept, the analysis and quantification of target compounds present in single cells of Allium cepa L was demonstrated.