The objective of present study was to determine the optimum level of papain in the diet of keureling fish (Tor tambra). The complete random design was utilized in this study. Six levels of papain dosage were tested in triplicates, i.e. 0 (control); 17.5 mg kg , 25.0 mg kg -1 and 27.5 mg kg -1 of feed. The experimental fish were fed the experimental diet two times a day at 8 AM and 5 PM at feeding level of 5% body weight for 90 days. The Anova test result showed that papain enzyme gave a significant effect on the weight gain, daily growth rate, specific growth rate, survival rate, feed conversion ratio and feed efficiency (P<0.05). The Duncan multi-rage test result showed that the higher values for all measured parameters were obtained at the dosage of 27.5 mg kg -1 . Therefore, it is concluded that the optimum dosage of papain enzyme for keureling fish was 27.5 mg kg -1 of feed.
Background
The objective of the present study was to determine the optimum dosage of probiotic in the diet of keureling fish (
Tor tambra) fry.
Methods
Lactobacillus casei from Yakult® was used as a starter, and enhanced with
Curcuma xanthorrhiza,
Kaempferia galanga and molasses. The mixture was fermented for 7 days prior to use as probiotic in a formulated diet containing 30% crude protein. Four levels of probiotic dosage; 0 ml kg
-1 (control), 5 ml kg
-1, 10 ml kg
-1 and 15 ml kg
-1 were tested in this study. The fish was fed twice a day at 08.00 AM and 06.00 PM at the ration of 5% body weight for 80 days.
Results
The results showed that growth performance and feed efficiency increased with increasing probiotic dosage in the diet from control (no probiotic) to 10 ml kg
-1 of probiotic dosage and then decreased when the dosage was increased up to 15 ml kg
-1.
Conclusions
The best values for all measured parameters were recorded at the dosage of 10 ml kg
-1. Therefore, it was concluded that the optimum dosage of enhanced probiotic for
T. tambra fry was 10 ml kg
-1 of feed.
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