Pituitary gonadotropes were studied in the adult male sand goby (Oxyeleotris marmoratus) during its annual reproductive cycle. Pituitary glands were taken from each of the 5 stages of testicular development: resting, developing, mature, spawning and spent. The pituitary glands were processed for Masson' s trichrome staining and immunocytochemistry using anti-chum salmon GTH Iβ and GTH IIβ antibodies. Most of the basophils in the proximal par distalis showed immunoreactivity to GTH IIβ. No cell showed immunoreactivity to GTH Iβ at any stage of testicular development. 2 ) and spent stage (29.16 ± 2.21 cell/mm 2 ). Based on the well known function of GTH, the presence of only GTH IIβ but not GTH Iβ in the pituitary gonadotropes suggested the involvement of GTH IIβ in spermatogenesis of the male sand goby. Moreover, the high number of GTH IIβ immunoreactive cells during testicular developing stage correlated with the maturation of sperm further confirmed the role of GTH IIβ in controlling cell proliferation during spermatogenesis.
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