Chronic lymphocyte leukemia (CLL) is a B‐cell malignancy resisted to apoptosis. Recently, some studies indicated that cytokines such as interleukin 27 (IL‐27) can reduce B‐cell proliferation. The aim of this study is to evaluate the mechanism underlying the proapoptotic effect of IL‐27 on B cells of patients with CLL in comparison with B cells of normal subjects. The effect of IL‐27 on the antitumor activity of natural killer (NK) and T cells was also evaluated. Peripheral blood mononuclear cells (PBMCs) were isolated from 35 patients with CLL and 15 normal subjects. B cells and PBMCs were cocultured with IL‐27 and B cells apoptosis to evaluate proliferation. Both messenger RNA and protein expression of IL‐27 and IL‐27 receptor were determined using flow cytometry and real‐time polymerase chain reaction analysis. To evaluate the apoptotic effect of IL‐27 on B cells of patients with CLL, Annexin V‐FITC and 7‐AAD (BioLegend) fluorescent dyes were used. In addition, the IL‐27 effect on activation of T cell and NK cell was determined by determining CD96 molecule expression. IL‐27 and IL‐27 receptor expression in patients with CLL was significantly lower than that of normal subjects (p < .05). IL‐27 enhanced apoptosis of B cells in patients with CLL (p < .05) but this effect was not significantly observed in B cells of normal subjects (p > .05). Consequently, IL‐27 reduced the proliferation of B cells and enhanced NK cell activity (p < .05). IL‐27, through inducing apoptosis, can exert an inhibitory effect on cancer B cells of CLL patients with minimal effect on normal B cells.
Introduction and Objective: Definitive diagnosis, tedious treatment methods, and long treatment duration are possible factors contributing to psychiatric disorders in cancerous patients, including anxiety and depression. Accordingly, this study aimed to investigate the effective factors on the occurrence of psychiatric disorders in newly diagnosed cancer patients. Methods: This descriptive-analytical study was conducted in Semnan, Iran, in 2018, in which 122 patients with cancer were included. In this study, the Hospital Anxiety and Depression Scale questionnaire was used to collect the required data. Frequency comparisons between the sub-groups were performed using the Chi-square test and, if necessary, Fisher’s exact test. Moreover, the T-test or Mann–Whitney U test was used to compare numerical variables. Results: In this study, the mean values of total depression and total anxiety among patients were 26.2% and 28.6%, respectively. According to the results, anxiety was observed in 74.3% of low-income people and 80% of females. Gender and income were significantly associated with anxiety in patients with cancer (p < 0/05). However, no significant association was found between depression and the variables of gender, age, level of education, and income (P > 0.05). Conclusion: The inclusion of psychiatric interventions in the treatment program of cancer patients can be effective in controlling psychiatric disorders, especially depression and anxiety.
Introduction: Anemia is one of the most common complications in dialysis patients. Deficiency of erythropoietin is the most common cause of anemia in dialysis patients. Chronic kidney disease (CKD) patients have a negative iron balance. Hepcidin is an antibacterial peptide that its synthesis is controlled by the iron surface and inflammatory markers and plays an important role in iron hemostasis in hemodialysis patients. In the presence of anemia, the level of hepcidin in normal individuals is reduced. Therefore, the normal level of hepcidin in CKD individuals is considered high due to their anemia level, while, the level of hepcidin is reduced after dialysis. Objectives: We aimed to study the level of hepcidin-25 in hemodialysis patients and its relationship with anemia and serum iron indices. Patients and Methods: In a cross-sectional study, 70 patients who fulfilled inclusion criteria enrolled in the study. Serum hepcidin 25 was measured by ELISA method. At the same time, blood samples were taken to measure other indices including iron, ferritin, C-reactive protein (CRP), total iron binding capacity (TIBC) and albumin. Hepcidin-25 levels compared with other mentioned parameters. Statistical analysis was done in SPSS 18.0 software. Results: No significant relationship between inflammatory cytokines, TIBC and CRP and erythrocyte sedimentation rate (ESR) with hepcidin levels was detected. Additionally, relationship between serum iron, ferritin and hepcidin levels was not significant (P > 0.05). Conclusion: Assessment of hepcidin 25 level for inflammation and iron status does not seem to be helpful for anemia management in hemodialysis patients.
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