Introducción. Diferentes características socio demográficas describen una disminución en el proceso de vacunación en niños originando rebrotes de enfermedades que se suponían controladas. Objetivos, comprobar la confiabilidad del instrumento de investigación para medir los factores que intervienen en el cumplimiento del calendario de vacunación en las madres con niños menores de 5 años. Materiales y métodos: esta investigación se empleo la metodología mixta, de corte transversal, exploratoria, descriptiva, en la cualitativa se usó la técnica fenomenológica con instrumentos diseñados por los investigadores y validado por juicio de expertos, Resultados. El instrumento de recolección de datos es validado con un puntaje promedio de pertinencia 89, validez de 90 y coherencia 89. Equivalente a 89.33 de confiabilidad, alfa de Cronbach de 0,07, Conclusiones. El instrumento al ser confiable está en condiciones de ser utilizado en investigaciones subsiguientes, teniendo en cuenta que la población a la que se aplicó reúne condiciones específicas de una área rural, dentro de los factores que modifican el cumplimiento del proceso de vacunación se recalcó las creencias de las madres del sector, quienes manifiestan un estado de inseguridad e incertidumbre al oponerse a la aplicación de vacunas a sus hijos, se detalla el estado emocional con tendencia a la depresión con rasgos de ansiedad de las madres como resultado de la actitud de su cultura de desconfianza. SUMMARYIntroduction: Different sociodemographic characteristics describe a decrease in the vaccination process in children, causing outbreaks of diseases that were supposed to be controlled. Objectives, to verify the reliability of the research instrument to measure the factors that intervene in the fulfillment of the vaccination schedule in mothers with children under 5 years of age. Materials and methods: this research used the mixed, cross-sectional, exploratory, descriptive methodology, in the qualitative one the phenomenological technique was used with instruments designed by the researchers and validated by expert judgment, Results. The data collection instrument is validated with an average relevance score of 89, validity of 90 and coherence of 89. Equivalent to 89.33 reliability, Cronbach's alpha of 0.07, Conclusions. The instrument, being reliable, is able to be used in subsequent investigations, taking into account that the population to which it was applied meets specific conditions of a rural area, within the factors that modify compliance with the vaccination process, beliefs of the mothers of the sector, who manifest a state of insecurity and uncertainty when opposing the application of vaccines to their children, the emotional state with a tendency to depression with anxiety traits of the mothers as a result of the attitude of their culture is detailed. of mistrust
HbA1c level reflects chronic glycemic status if reliable tests are used. The HbA1c testing improved with the efforts of many national and international organizations; however, in some regions, high performing assays might not be readily available. Here we report a comparison study of two immunoassay methods with a high-performance liquid chromatography (HPLC) assay, all of them available in Ecuador. We studied 114 fresh whole-blood samples from adults with normal and pathological HbA1c levels. HbA1c was measured in all samples by DCA-Vantage point-of-care analyzer, I-Chroma portable fluorescent scanner immunoassay and BioRad Variant II Turbo HPLC. Blood samples with variants of hemoglobin were excluded. HbA1c values were expressed in National Glycohemoglobin Standardization Program percentages and mmol/mol, as mean ± standard deviation. Results: HbA1c measurements by HPLC and DCA-Vantage were similar: 6.3±1.7% (45±18.6 mmol/mol) vs. 6.3±1.8% (45±19.7 mmol/mol), respectively, P=0.057, while HbA1c values by I-Chroma were lower than HPLC, 5.8±1.9% (40±20.8 mmol/mol), P<0.001. Interestingly, the coefficient of variation was below 2% for high and low HbA1c levels, in all methods studied, demonstrating high precision. HbA1c values by HPLC and DCA-Vantage were highly correlated, Kendall's Correlation Coefficient [KCC]: 0.817, whereas the correlation among HPLC and I-chroma was weak, KCC: 0.294. The mean bias between DCA-Vantage and HPLC was -0.02±0.29% (-0.2±3.2 mmol/mol), 95% Confidence Interval of the differences (CID) [0.55%, -0.60%] (6.0 mmol/mol, -6.6 mmol/mol) while for I-Chroma and HPLC the mean bias was -0.50±1.62% (-5.5±17.7 mmol/mol), 95% CID [2.74%, -3.74 %] (30 mmol/mol, -40.9 mmol/mol). Conclusion: HbA1c results by DCA-Vantage were comparable to HPLC, showing a good correlation, an appropriate precision and a low bias. While I-Chroma assay was precise but inaccurate. Our results suggest a significant need for improvement in HbA1c immunoassays currently in use in our country, Ecuador. Disclosure B.J. Pazmiño Gomez: None. F.E. Vera Lorenti: None. M.E. Guadalupe Vargas: None. G.M. Alvarez Condo: None. D. Veron: None. A.G. Cercado: None. E.I. Rodas Neira: None. B. Bahar: None. D. Veron: None. Funding Ministry of Higher Education, Science, Technology and Innovation, Ecuador/Prometeo Project/State University of Milagro, Ecuador (UNEMI-OCAS-SO-27092013-N?26 DV2013?2014, UNEMI-OCAS-SO-03072014-N8-DV2014-2015, UNEMI-OCAS-SO-30052016-N5-DV2015-2017)
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