Abstract. Atmospheric warming is intensifying glacier melting and glacial-lake development in High Mountain Asia (HMA), and this could increase glacial-lake outburst flood (GLOF) hazards and impact water resources and hydroelectric-power management. There is therefore a pressing need to obtain comprehensive knowledge of the distribution and area of glacial lakes and also to quantify the variability in their sizes and types at high resolution in HMA. In this work, we developed an HMA glacial-lake inventory (Hi-MAG) database to characterize the annual coverage of glacial lakes from 2008 to 2017 at 30 m resolution using Landsat satellite imagery. Our data show that glacial lakes exhibited a total area increase of 90.14 km2 in the period 2008–2017, a +6.90 % change relative to 2008 (1305.59±213.99 km2). The annual increases in the number and area of lakes were 306 and 12 km2, respectively, and the greatest increase in the number of lakes occurred at 5400 m elevation, which increased by 249. Proglacial-lake-dominated areas, such as the Nyainqêntanglha and central Himalaya, where more than half of the glacial-lake area (summed over a 1∘ × 1∘ grid) consisted of proglacial lakes, showed obvious lake-area expansion. Conversely, some regions of eastern Tibetan mountains and Hengduan Shan, where unconnected glacial lakes occupied over half of the total lake area in each grid, exhibited stability or a slight reduction in lake area. Our results demonstrate that proglacial lakes are a main contributor to recent lake evolution in HMA, accounting for 62.87 % (56.67 km2) of the total area increase. Proglacial lakes in the Himalaya ranges alone accounted for 36.27 % (32.70 km2) of the total area increase. Regional geographic variability in debris cover, together with trends in warming and precipitation over the past few decades, largely explains the current distribution of supraglacial- and proglacial-lake area across HMA. The Hi-MAG database is available at https://doi.org/10.5281/zenodo.4275164 (Chen et al., 2020), and it can be used for studies of the complex interactions between glaciers, climate and glacial lakes, studies of GLOFs, and water resources.

Big data has been a focus of research in science, technology, economics, and social studies. Many countries have already incorporated big data research into their national strategies. This paper elaborates upon the origin, connotation, and development of big data from both a spatial and temporal perspective. It proposes that scientific big data will become a new solution in scientific research as the paradigm changes from being model-driven to data-driven. This paper defines the concept of ''scientific big data'' and proposes strategies for solving ''big data problems''. Theoretical frameworks and data systems for Digital Earth are discussed with a clear conclusion that scientific big data is a prominent feature of Digital Earth. As an example, spatial cognition of the formation mechanism of China's Heihe-Tengchong Line-a geo-demographic demarcation line dividing China into two parts-is discussed within the context of big data computation and analysis for Digital Earth.

Chitooligomers or chitooligosaccharides (COS) are elicitors that bind to the plasma membrane (PM) and elicit various defense responses. However, the PM-bound proteins involved in elicitor-mediated plant defense responses still remain widely unknown. In order to get more information about PM proteins involved in rice defense responses, we conducted PM proteomic analysis of the rice suspension cells elicited by COS. A total of 14 up-or downregulated protein spots were observed on 2-D gels of PM fractions at 12 h and 24 h after COS incubation. Of them, eight protein spots were successfully identified by MS (mass spectrography) and predicted to be associated to the PM and function in plant defense, including a putative PKN/PRK1 protein kinase, a putative pyruvate kinase isozyme G, a putative zinc finger protein, a putative MAR-binding protein MFP1, and a putative calcium-dependent protein kinase. Interestingly, a COS-induced pM5-like protein was identified for the first time in plants, which is a transmembrane nodal modulator in transforming growth factor-β β β β β (TGFβ β β β β) signaling in vertebrates. We also identified two members of a rice polyprotein family, which were up-regulated by COS. Our study would provide a starting point for functionality of PM proteins in the rice basal defense.

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A series of renewable C -C triketones with repeating [COCH CH ] units were synthesized in high carbon yields (ca. 90 %) by the aqueous-phase hydrogenation of the aldol-condensation products of 5-hydroxylmethylfurfural (HMF) and ketones over an Au/TiO catalyst. Compared with the reported routes, this new route has many advantages such as being environmentally friendly, having fewer steps, using a cheaper and reusable catalyst, etc. The triketones as obtained can be used as feedstocks in the production of conducting or semi-conducting polymers. Through a solvent-free intramolecular aldol condensation over solid-base catalysts, the triketones were selectively converted to diketones, which can be used as intermediates in the synthesis of useful chemicals or polymers. As another application, the tri- and diketones can also be utilized as precursors for the synthesis of jet-fuel range branched cycloalkanes with low freezing points (224-248 K) and high densities (ca. 0.81 g mL ).

Mycobacterium tuberculosis infection remains a major threat to human health worldwide. Drug treatments against tuberculosis (TB) induce expression of several mycobacterial proteins, including IniA, but its structure and function remain poorly understood. Here, we report the structures of Mycobacterium smegmatis IniA in both the nucleotide-free and GTP-bound states. The structures reveal that IniA folds as a bacterial dynamin-like protein (BDLP) with a canonical GTPase domain followed by two helix-bundles (HBs), named Neck and Trunk. The distal end of its Trunk domain exists as a lipid-interacting (LI) loop, which binds to negatively charged lipids for membrane attachment. IniA does not form detectable nucleotide-dependent dimers in solution. However, lipid tethering indicates nucleotide-independent association of IniA on the membrane. IniA also deforms membranes and exhibits GTP-hydrolyzing dependent membrane fission. These results confirm the membrane remodeling activity of BDLP and suggest that IniA mediates TB drug-resistance through fission activity to maintain plasma membrane integrity.

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An immune response is triggered in host cells when host receptors recognize conserved molecular motifs, pathogen-associated molecular patterns (PAMPs), such as βglucans, and chitin at the cell surface of a pathogen. Effectortriggered immunity occurs when pathogens deliver effectors into the host cell to suppress the first immune signaling. Using a differential proteomic approach, we identified an array of proteins responding to aflatoxins in cotyledons of peanut (Arachis hypogaea) infected with aflatoxin-producing (toxigenic) but not nonaflatoxin-producing (atoxigenic) strains of Aspergillus f lavus. These proteins are involved in immune signaling and PAMP perception, DNA and RNA stabilization, induction of defense, innate immunity, hypersensitive response, biosynthesis of phytoalexins, cell wall responses, peptidoglycan assembly, penetration resistance, condensed tannin synthesis, detoxification, and metabolic regulation. Gene expression analysis confirmed the differential abundance of proteins in peanut cotyledons supplemented with aflatoxins, with or without infection with the atoxigenic strain. Similarly, peanut germination and A. f lavus growth were altered in response to aflatoxin B1. These findings show an additional immunity initiated by aflatoxins. With the PAMP-and effector-triggered immune responses, this immunity constitutes the third immune response of the immune system in peanut cotyledon cells. The system is also a threegrade coevolution of plant−pathogen interaction.