In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has emerged as the most common method to image deep within scattering biological tissue. This technique, however, requires high-power pulsed lasers that are both expensive and difficult to integrate into compact portable systems. Using a combination of theoretical and experimental techniques, we show that if the excitation path length can be minimized, selective plane illumination microscopy (SPIM) can image nearly as deep as 2PM without the need for a high-powered pulsed laser. Compared to other single-photon imaging techniques like epifluorescence and confocal microscopy, SPIM can image more than twice as deep in scattering media ( ? 10 times the mean scattering length). These results suggest that SPIM has the potential to provide deep imaging in scattering media in situations in which 2PM systems would be too large or costly.
Insight into the mechanisms driving protein-polymer interactions is constantly improving due to advances in experimental and computational methods. In this study, we used super-temporal-resolved microscopy (STReM) to study the interfacial kinetics of a globular protein, α-lactalbumin (α-LA), adsorbing at the water-nylon 6,6 interface. The improved temporal resolution of STReM revealed that residence time distributions involve an additional step in the desorption process. Increasing the ionic strength in the bulk solution accelerated the desorption rate of α-LA, attributed to adsorption-induced conformational changes. Ensemble circular dichroism measurements were used to support a consecutive reaction mechanism. Without the improved temporal resolution of STReM, the desorption intermediate was not resolvable, highlighting both STReM's potential to uncover new kinetic mechanisms and the continuing need to push for better time and space resolution.
Plasmonic
photocatalytic processes typically use the interaction
of light with metallic nanoparticles to drive chemical reactions on
their surfaces. Here we show that a plasmonic photocatalyst can also
induce a reaction on an adjacent material. A combination of spontaneous
H2 dissociation and plasmon-induced H desorption from tilted
palladium (Pd) nanocones yields reactive H atoms which, in the direct
vicinity of a graphene monolayer, results in its local hydrogenation.
The conversion of pristine to hydrogenated graphene, a semiconductor,
is detectable by visible local fluorescence of the hydrogenated regions
of the graphene sheet, as well as by Raman spectroscopic analysis.
These results may lead to new approaches for local, light-driven functionalization
of graphene and other 2D materials and for precision patterning of
functional devices.
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